Identity of human epidermal growth factor (EGF) receptor with glycoprotein SA-7: evidence for differential phosphorylation of the two components of the EGF receptor from A431 cells.
Identity of human epidermal growth factor (EGF) receptor with glycoprotein SA-7: evidence for differential phosphorylation of the two components of the EGF receptor from A431 cells.
复制标题
人表皮生长因子 (EGF) 受体与糖蛋白 SA-7 的同一性：A431 细胞 EGF 受体两种成分差异磷酸化的证据。
DOI：
10.1073/pnas.79.16.5026
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发表时间：
1982
影响因子：
11.1
通讯作者：
Knowles,BB
中科院分区：
文献类型：
--
作者：
Carlin,CR;Knowles,BB
利用人7号染色体体细胞杂交种的抗血清，鉴定了人7号染色体编码的一个165千道尔顿（kDal）表面糖蛋白。我们现在提出的证据表明，SA-7是人类表皮生长因子（EGF）的受体，这些抗血清识别人类特异性决定因子。编码人类EGF受体的基因位于7号染色体的p12至p22区域。在用这些抗血清免疫沉淀放射性标记细胞提取物后，我们鉴定了A431细胞的145-kDal/165-kDal EGF受体双链。我们发现一种具有内源性激酶活性的蛋白质与A431受体双联体结合，双联体的两个组分都含有磷酸酪氨酸和磷酸苏氨酸，165-kDal组分也含有磷酸丝氨酸。此外，尽管受体双偶体的每个组成部分的平均pI为7，但两者都显示出电荷异质性，并且似乎具有独特的电荷异构体。讨论了A431 EGF受体两组分之间的关系。
A 165-kilodalton (kDal) surface glycoprotein encoded by human chromosome 7 (SA-7) had been characterized by using antisera raised against human chromosome 7-containing somatic cell hybrids. We now present evidence that SA-7 is the human receptor for epidermal growth factor (EGF) and that these antisera recognize human-specific determinants. The gene coding for the human EGF receptor is localized to the p12 to p22 region of chromosome 7. We have characterized the 145-kDal/165-kDal EGF receptor doublet of A431 cells after immunoprecipitation of radiolabeled cell extracts with these antisera. We find that a protein with endogenous kinase activity copurifies with the A431 receptor doublet and that both components of the doublet contain phosphotyrosine and phosphothreonine and the 165-kDal component contains phosphoserine as well. Further, although each component of the receptor doublet has an average pI of 7, both display charge heterogeneity and appear to have unique charge isomers. The relationship between the two components of the A431 EGF receptor is discussed.