Characterization of infectious hepatitis C virus from liver-derived cell lines
Characterization of infectious hepatitis C virus from liver-derived cell lines
复制标题
DOI：
10.1016/j.bbrc.2008.10.063
复制
发表时间：
2008-12-19
影响因子：
3.1
通讯作者：
Wakita, Takaji
中科院分区：
文献类型：
--
作者：
Akazawa, Daisuke;Date, Tomoko;Wakita, Takaji
从JFH-1株有效生产感染性HCV仅限于Huh 7细胞系及其衍生物。然而，这种限制所涉及的因素尚不清楚。在这项研究中，我们研究了其他肝源性细胞系的感染性HCV的生产，并表征了所产生的病毒。获得携带JFH-1全基因组复制子的Huh 7、HepG 2和IMY-N9的克隆。每个细胞克隆的上清液显示出对初始Huh 7的感染性。然后通过蔗糖密度梯度表征每个感染性上清液。对于所有的细胞系，HCV核心蛋白和RNA的主峰在约1.15 g/mL的浮力密度下显示。然而，来自IMY-N9的上清液与Huh 7的上清液在1.15 μ g/mL的核心：RNA比率方面不同，并且在较低密度下也观察到显著峰。从不同细胞系产生的病毒颗粒可能具有不同的特征。(C)2008年爱思唯尔公司All rights reserved.
The efficient production of infectious HCV from the JFH-1 strain is restricted to the Huh7 cell line and its derivatives. However, the factors involved in this restriction are unknown. In this study, we examined the production of infectious HCV from other liver-derived cell lines, and characterized the produced viruses. Clones of the Huh7, HepG2, and IMY-N9, harboring the JFH-1 full-genomic replicon, were obtained. The supernatant of each cell clone exhibited infectivity for naive Huh7. Each infectious supernatant was then characterized by sucrose density gradient. For all of the cell lines, the main peak of the HCV-core protein and RNA exhibited at approximately 1.15 g/mL of buoyant density. However, the supernatant from the IMY-N9 differed from that of Huh7 in the ratio of core:RNA at 1.15 g/mL and significant peaks were also observed at lower density. The virus particles produced from the different cell lines may have different characteristics. (C) 2008 Elsevier Inc. All rights reserved.