EFFECT OF MOUSE PERITONEAL-MACROPHAGES ON SCRAPIE INFECTIVITY DURING EXTENDED INVITRO INCUBATION
EFFECT OF MOUSE PERITONEAL-MACROPHAGES ON SCRAPIE INFECTIVITY DURING EXTENDED INVITRO INCUBATION
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DOI：
10.1159/000149289
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发表时间：
1982-01-01
期刊：
影响因子：
4.6
通讯作者：
CALLAHAN, SM
中科院分区：
文献类型：
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作者：
CARP, RI;CALLAHAN, SM
将小鼠腹腔巨噬细胞在体外37 ℃暴露于羊瘙痒病（ME 7株）[小鼠]脑匀浆2小时。C.通过分析羊瘙痒病孵育期并将其值与延长（1-28天）体外孵育后获得的值进行比较，测定样品的感染性。羊瘙痒病因子-巨噬细胞混合物的羊瘙痒病孵育期已经历了延长的体外孵育时间长于混合物暴露于羊瘙痒病因子2小时后测定。当通过在暴露于脑匀浆2小时后洗涤细胞来消除残留的羊瘙痒病时，羊瘙痒病潜伏期的差异更加显著。在没有细胞的情况下，ME 7体外孵育后，羊瘙痒症潜伏期也增加;变化小于羊瘙痒症因子-巨噬细胞混合物中观察到的变化。在细胞暴露于羊瘙痒病因子后被紫外线照射破坏的培养物中，羊瘙痒病的感染性比未经处理的暴露于羊瘙痒病的巨噬细胞的可比培养物中更强。因此，羊瘙痒病感染性随着暴露于羊瘙痒病的巨噬细胞的延长孵育而降低，并且巨噬细胞在体外明显可以抑制羊瘙痒病因子。
Mouse peritoneal macrophages were exposed to scrapie (ME7 strain) [mouse] brain homogenate in vitro for 2 h at 37.degree. C. The samples were assayed for infectivity by analysis of scrapie incubation periods and their values compared to those obtained after extended (1-28 days) in vitro incubation. The scrapie incubation periods for scrapie agent-macrophage mixtures which had undergone extended in vitro incubation were longer than for mixtures assayed after a 2 h exposure to the scrapie agent. The difference in scrapie incubation periods was more dramatic when residual scrapie was eliminated by washing the cells after the 2 h exposure to brain homogenate. The scrapie incubation period also increased following in vitro incubation of ME7 in the absence of cells; the changes were less than those observed for scrapie agent-macrophage mixtures. In a culture in which cells had been destroyed by UV irradiation after exposure to the scrapie agent, there was more scrapie infectivity than in a comparable culture of untreated, scrapie-exposed macrophages. Thus, scrapie infectivity decreases with extended incubation of scrapie-exposed macrophages and macrophages evidently can inactivate the scrapie agent in vitro.