Identification and expression analysis of IRAK-4 cDNA and its gene from the ayu Plecoglossus altivelis altivelis
Identification and expression analysis of IRAK-4 cDNA and its gene from the ayu Plecoglossus altivelis altivelis
复制标题
DOI：
10.1007/s12562-015-0942-z
复制
发表时间：
2015
影响因子：
1.9
通讯作者：
Kyuma Suzuki;S. Izumi;Hideki Tanaka;T. Katagiri
中科院分区：
文献类型：
--
作者：
Kyuma Suzuki;S. Izumi;Hideki Tanaka;T. Katagiri
白介素 1 受体相关激酶 4 (IRAK-4) 是哺乳动物针对传染病的先天和适应性防御系统的重要组成部分。在本研究中，克隆并分析了 ayuPlecoglossus altivelis altivelisIRAK-4 cDNA 和基因组 DNA。 ayu IRAK-4 cDNA 的开放阅读框编码 463 个氨基酸，包括哺乳动物 IRAK-4 基序的死亡结构域和丝氨酸/苏氨酸蛋白激酶结构域。香鱼IRAK-4基因包含11个外显子，并且被发现与迄今为止描述的脊椎动物IRAK-4基因非常相似。 Ayu IRAK-4 mRNA 在所有组织中表达，主要在鳃、心脏、肠、整个肾脏和脾脏中表达。在用嗜冷黄杆菌浸泡攻击 12 小时后，与对照相比，全血中 ayu IRAK-4 mRNA 表达上调（21.6 倍）。目前的研究表明香鱼IRAK-4可能参与免疫反应。
Interleukin-1 receptor-associated kinase 4 (IRAK-4) is an important component of the mammalian innate and adaptive defense system against infectious disease. In this study, ayuPlecoglossus altivelis altivelisIRAK-4 cDNA and genomic DNA were cloned and analyzed. The open reading frame of ayu IRAK-4 cDNA encodes 463 amino acids, including a death domain and a serine/threonine protein kinase domain of the mammalian IRAK-4 motif. The ayu IRAK-4 gene comprises 11 exons, and was found to be very similar to vertebrate IRAK-4 genes described thus far. Ayu IRAK-4 mRNA was expressed in all tissues, and predominantly in the gill, heart, intestine, whole kidney, and spleen. At 12 h after immersion challenge withFlavobacterium psychrophilum, ayu IRAK-4 mRNA expression in the whole blood was up-regulated (21.6-fold) compared to the control. The present study suggests that ayu IRAK-4 may be involved in the immune response.