Cytotoxicity analysis of pre- and post-hurricane harvey soil samples collected from greater houston bayous
Cytotoxicity analysis of pre- and post-hurricane harvey soil samples collected from greater houston bayous
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DOI：
10.1016/j.ecoenv.2021.112600
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发表时间：
2021-08-05
影响因子：
6.8
通讯作者：
Sridhar, Balaji Bhaskar Maruthi
中科院分区：
文献类型：
--
作者：
Keita, Djene;Shishodia, Shishir;Sridhar, Balaji Bhaskar Maruthi
快速的城市化、人为污染和频繁的洪水事件正在影响休斯顿沿着河流和海湾的土壤和水质。土壤作为重金属和营养物质的汇和库，影响人类和动物的健康。本研究的目的是1）分析河口河漫滩表层土壤样品的金属和营养浓度对肠道细胞毒性的影响; 2）评估土壤污染对结肠癌（HT-29）和正常结肠上皮（CCD 841 CoN）细胞系细胞活力的时空差异。为了评估得克萨斯州哈里斯县六个Bayous（Brays、布法罗、霍尔斯、狩猎、格林和白色橡树Bayous）飓风前和飓风后（夏季和秋季）条件下的土壤污染，对土壤提取物进行了体外生物测定分析。MTT试验确定，随着河口土壤浓度从12.5%增加到100%，CCD 841 CoN和HT-29细胞的活力显著降低，在夏季和秋季的所有采样位置。在所有的海湾中，CCD 841 CoN细胞的活力在夏季和秋季遵循的模式白色橡树>绿色>大厅>布雷斯河口，其中暴露于白色橡树土壤的细胞的活力是3 - 4倍高于暴露于布雷斯河口土壤在100%的土壤浓度。HT-29细胞在两个季节中的存活率遵循Greens>白色Oak>霍尔斯>布雷斯河口的模式，其中暴露于Greens Bayou土壤的细胞的存活率比暴露于布雷斯河口土壤的细胞在100%浓度下高3 - 4倍。较高浓度的金属和营养物质，如P，Zn，Cd和Cu可能有助于在Brays Bayou样品相比，其他位置的显着的细胞致死率。
Rapid urbanization, anthropogenic pollution and frequent flooding events are affecting the soil and water quality along the streams and bayous of Houston. Soil acts as sink and reservoir of heavy metals and nutrients affecting human and animal health. The objectives of the study are 1) to analyze the effects of the metal and nutrient concentration of bayou flood plain surface soil samples on the gut cell cytotoxicity and 2) to evaluate the spatial and temporal difference in soil contamination on cell viability of colon cancer (HT-29) and normal colon epithelial (CCD 841 CoN) cell lines. To evaluate soil contamination between pre- and post-hurricane (Summer and Fall) conditions in six Bayous (Brays, Buffalo, Halls, Hunting, Greens and White Oak Bayous) of Harris County, Texas, in vitro bioassay analysis was applied to soil extracts. The MTT assay determined that, with increase in concentration of Bayou soil from 12.5% to 100%, the viability of CCD 841 CoN and HT-29 cells decreased significantly, across all sampling locations during both summer and fall seasons. Among all the bayous, the viability of CCD 841 CoN cells in summer and fall followed the pattern of White Oak > Greens > Halls > Brays Bayou, where the viability of cells exposed to White Oak soils was 3-4 times higher than cells exposed to Brays Bayou soil at 100% soil concentration. The viability of HT-29 cells in both seasons followed the pattern of Greens > White Oak > Halls > Brays Bayou, where the viability of cells exposed to Greens Bayou soil was more than 3-4 times higher than the cells exposed to Brays Bayou soil at 100% concentration. The higher concentration of metals and nutrients such as P, Zn, Cd, and Cu might have contributed to the significant cell lethality in Brays Bayou samples compared to other locations.