Effects of dehydroepiandrosterone on proliferation and differentiation of chromaffin progenitor cells
Effects of dehydroepiandrosterone on proliferation and differentiation of chromaffin progenitor cells
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DOI：
10.1016/j.mce.2010.11.028
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发表时间：
2011-04-10
影响因子：
4.1
通讯作者：
Ehrhart-Bornstein, Monika
中科院分区：
文献类型：
--
作者：
Chung, Kuei-Fang;Qin, Nan;Ehrhart-Bornstein, Monika
在许多物种中，产生脱氢表雄酮的肾上腺皮质网状带和肾上腺髓质是直接接触的并且高度混合。这导致局部高浓度脱氢表雄酮对肾上腺髓质产生潜在的强烈旁分泌影响。脱氢表雄酮具有神经保护特性，可增加神经干细胞增殖和神经发生。因此，我们的目的是确定其对嗜铬祖细胞增殖和分化的影响。此前，我们成功地从球形培养物（所谓的色球）中的牛肾上腺髓质中分离出了嗜铬祖细胞。用高浓度（100 μM）脱氢表雄酮对色球进行 7 天的处理会阻碍嗜铬祖细胞的增殖。在分化条件下，在视黄酸存在下，脱氢表雄酮会增加酪氨酸羟化酶并减少多巴胺-β-羟化酶 mRNA 表达。此外，多巴胺含量有增加的趋势。因此，建议脱氢表雄酮/视黄酸诱导嗜铬祖细胞的多巴胺能分化。此外，胎儿和成人肾上腺中存在的高浓度脱氢表雄酮可能在肾上腺髓质细胞增殖和分化中发挥重要作用。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
Dehydroepiandrosterone producing adrenocortical zona reticularis and the adrenal medulla are in direct contact and are highly intermingled in many species. This results in potentially strong paracrine influences of high local dehydroepiandrosterone concentrations on the adrenal medulla. Dehydroepiandrosterone has neuroprotective properties and increases neural stem cell proliferation and neurogenesis. Therefore, we aimed to establish its effects on chromaffin progenitor cell proliferation and differentiation. Previously, we successfully isolated chromaffin progenitors from bovine adrenal medulla in spherical cultures, so-called chromospheres. Seven days treatment of chromospheres with dehydroepiandrosterone at high concentrations (100 mu M) hampered proliferation of chromaffin progenitors. Under differentiation conditions, dehydroepiandrosterone in the presence of retinoic acid, increased tyrosine hydroxylase and decreased dopamine-beta-hydroxylase mRNA expression. In addition, there was a tendency to increase dopamine contents. Dehydroepiandrosterone/retinoic acid is therefore suggested to induce dopaminergic differentiation from chromaffin progenitor cells. Furthermore, the high dehydroepiandrosterone concentrations present in the fetal and adult adrenal may play an important role in adrenomedullary cell proliferation and differentiation. (C) 2010 Elsevier Ireland Ltd. All rights reserved.