Kinetics of IL-7 and IL-15 levels after allogeneic peripheral blood stem cell transplantation following nonmyeloablative conditioning.
Kinetics of IL-7 and IL-15 levels after allogeneic peripheral blood stem cell transplantation following nonmyeloablative conditioning.
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DOI：
10.1371/journal.pone.0055876
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Baron F
中科院分区：
文献类型：
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作者：
De Bock M;Fillet M;Hannon M;Seidel L;Merville MP;Gothot A;Beguin Y;Baron F
我们分析了 70 名接受非清髓性预处理后接受外周血干细胞治疗的患者的 IL-7 和 IL-15 水平的动力学。在调理前和移植后大约每周一次直至第 100 天获取 EDTA 抗凝血浆和血清样本。将样本等分并在收集后 3 小时内保存在 -80°C 下，直至测量细胞因子。通过 ELISA 测量 IL-7 和 IL-15 水平。在前 100 天中，IL-7 血浆水平中位数保持在 6 pg/L 以下，尽管 IL-7 血浆水平在第 7 天（5.1 pg/mL，P = 0.002）、第 14 天（5.2 pg/mL，P<0.001）和 28 天（5.1 pg/mL，P = 0.03）（但此后）显着高于移植前（中值为 3.8 pg/mL）。第 7 天（12.5 pg/mL，P<0.001）、第 14 天（10.5 pg/mL，P<0.001）和 28 天（6.2 pg/mL，P<0.001）的中位 IL-15 血清水平显着高于移植前（中位值为 2.4 pg/mL）。重要的是，移植后第 7 天或第 14 天的 IL-7 和 IL-15 水平并不能预测 II-IV 级急性 GVHD。这些数据表明，非清髓性移植后 IL-7 和 IL-15 水平仍然相对较低，并且非清髓性移植后早期的 IL-7 和 IL-15 水平不能预测急性 GVHD。
We analysed kinetics of IL-7 and IL-15 levels in 70 patients given peripheral blood stem cells after nonmyeloablative conditioning. EDTA-anticoagulated plasma and serum samples were obtained before conditioning and about once per week after transplantation until day 100. Samples were aliquoted and stored at −80°C within 3 hours after collection until measurement of cytokines. IL-7 and IL-15 levels were measured by ELISAs. Median IL-7 plasma levels remained below 6 pg/L throughout the first 100 days, although IL-7 plasma levels were significantly higher on days 7 (5.1 pg/mL, P = 0.002), 14 (5.2 pg/mL, P<0.001), and 28 (5.1 pg/mL, P = 0.03) (but not thereafter) than before transplantation (median value of 3.8 pg/mL). Median IL-15 serum levels were significantly higher on days 7 (12.5 pg/mL, P<0.001), 14 (10.5 pg/mL, P<0.001), and 28 (6.2 pg/mL, P<0.001) than before transplantation (median value of 2.4 pg/mL). Importantly, IL-7 and IL-15 levels on days 7 or 14 after transplantation did not predict grade II–IV acute GVHD. These data suggest that IL-7 and IL-15 levels remain relatively low after nonmyeloablative transplantation, and that IL-7 and IL-15 levels early after nonmyeloablative transplantation do not predict for acute GVHD.