Expression pattern of the murine LIM class homeobox gene Lhx3 in subsets of neural and neuroendocrine tissues
Expression pattern of the murine LIM class homeobox gene Lhx3 in subsets of neural and neuroendocrine tissues
复制标题
小鼠 LIM 类同源盒基因 Lhx3 在神经和神经内分泌组织亚群中的表达模式
作者：
A. Zhadanov;S. Bertuzzi;M. Taira;I. Dawid;H. Westphal
从小鼠垂体cDNA文库中分离的小鼠Lhx 3 cDNA编码LIM型同源结构域蛋白，其含有两个串联重复的LIM结构域和同源结构域。小鼠Lhx 3和非洲爪蟾Xlim-3基因之间的预测氨基酸序列的同一性在LIM结构域1和2以及同源结构域中分别为80%、95%和97%，在整个蛋白质中为84%。5′-RACE程序和基因组克隆显示，两个不同的N-末端序列来自两个不同的外显子1a和1b。外显子1a编码与Xlim-3相似的序列，而外显子1b编码不同的N末端。Lhx 3基因可能有两个转录起始位点。在胚胎移植后第9.5天，用全胚原位杂交法在Rathke囊和闭合的神经管中检测到Lhx 3转录本。在随后的发展中，Lhx 3的表达被观察到在前和中间，但不是在垂体后叶，并在腹侧后脑和脊髓。成体组织的北方印迹分析表明，Lhx 3 mRNA持续存在于垂体中。Lhx 3的表达模式在非洲爪蟾和小鼠之间是非常保守的，强调了该基因作为发育调节因子的功能重要性。一些已建立的垂体来源的细胞系表达Lhx 3，因此构成了一个有用的工具，为进一步研究Lhx 3基因的功能。© 1995 Wiley利斯公司
Murine Lhx3 cDNA isolated from the mouse pituitary cDNA library encodes a LIM‐type homeodomain protein that contains two tandemly repeated LIM domains and the homeodomain. The identities of predicted amino acid sequences between the mouse Lhx3 and Xenopus Xlim‐3 genes are 80, 95, and 97% in the LIM domains 1 and 2, and the homeodomain, respectively, and 84% in the entire protein. 5′‐RACE procedures and genomic cloning revealed that two distinct N‐terminal sequences arise from two different exons 1a and 1b. Exon 1a encodes a sequence similar to that of Xlim‐3, whereas exon 1b encodes a different N‐terminus. It is likely that there are two transcription initiation sites in the Lhx3 gene. The Lhx3 transcripts were detected by whole mount in situ hybridization as early as day E9.5 post coitum in Rathke's pouch and the closing neural tube. During subsequent development, Lhx3 expression was observed in the anterior and intermediate but not in the posterior lobes of the pituitary, and in the ventral hindbrain and spinal cord. Northern blot analysis of adult tissues showed that Lhx3 mRNA persists in the pituitary. The expression pattern of Lhx3 is well conserved between Xenopus and mouse, underscoring the functional importance of this gene as a regulator of development. A number of established cell lines of pituitary origin express Lhx3 and therefore constitute a useful tool for further study of Lhx3 gene function. © 1995 Wiley‐Liss, Inc.
影响因子：
--
作者：
L. Swanson
通讯作者：
L. Swanson
影响因子：
56.9
作者：
CHALFIE, M;AU, M
通讯作者：
AU, M
使用 DNA 聚合酶链式反应进行同源性探测：从多个物种中分离编码琥珀酸脱氢酶铁硫蛋白的部分 cDNA 或基因组克隆。
DOI：
10.1073/pnas.86.6.1934
发表时间：
1989
影响因子：
11.1
作者：
Gould,SJ;Subramani,S;Scheffler,IE
通讯作者：
Scheffler,IE