The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168
The structures of the lipooligosaccharide and capsule polysaccharide of Campylobacter jejuni genome sequenced strain NCTC 11168
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DOI：
10.1046/j.1432-1033.2002.03201.x
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发表时间：
2002-11-01
期刊：
影响因子：
--
通讯作者：
Monteiro, MA
中科院分区：
文献类型：
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作者：
St Michael, F;Szymanski, CM;Monteiro, MA
空肠弯曲杆菌感染是人类胃肠炎的主要原因之一，并被怀疑是格林-巴利综合征和米勒-费舍尔综合征的前兆。最近，C.空肠NCTC 11168。本研究对C.空肠NCTC 11168。脂寡糖显示出与人神经节苷脂的GM 1a和GM 2糖表位类似的糖结构（如下所示）[图表]高M-r胶囊多糖由bbeta-D-Ribp、beta-D-GalfNAc、a-D-GlcpA 6（NGro）（一种在C-6用2-氨基-2-脱氧甘油酰胺化的糖醛酸）和作为侧支的6-O-甲基-D-甘油-a-L-葡萄糖-吡喃庚糖组成（如下所示）：[图表]这里提供的结构信息将有助于鉴定和表征参与这些结构生物合成的特定酶，并可能导致发现潜在的治疗靶点。此外，碳水化合物结构与基因互补的相关性将有助于阐明这些表面碳水化合物在C.空肠发病机制
Campylobacter jejuni infections are one of the leading causes of human gastroenteritis and are suspected of being a precursor to Guillain-Barre and Miller-Fisher syndromes. Recently, the complete genome sequence of C. jejuni NCTC 11168 was described. In this study, the molecular structure of the lipooligosaccharide and capsular polysaccharide of C. jejuni NCTC 11168 was investigated. The lipooligosaccharide was shown to exhibit carbohydrate structures analogous to the GM1a an GM2 carbohydrate epitopes of human gangliosides (shown below)[GRAPHICS]The high M-r capsule polysaccharide was composed of bbeta-D-Ribp, beta-D-GalfNAc, alpha-D-GlcpA6(NGro), a uronic acid amidated with 2-amino-2-deoxyglycerol at C-6, and 6-O-methyl-D-glycero-alpha-L-gluco-heptopyranose as a side-branch (shown below):[GRAPHICS]The structural information presented here will aid in the identification and characterization of specific enzymes that are involved in the biosynthesis of these structures and may lead to the discovery of potential therapeutic targets. In addition, the correlation of carbohydrate structure with gene complement will aid in the elucidation of the role of these surface carbohydrates in C. jejuni pathogenesis.