Normal assembly of 60 S ribosomal subunits is required for the signaling in response to a secretory defect in Saccharomyces cerevisiae
Normal assembly of 60 S ribosomal subunits is required for the signaling in response to a secretory defect in Saccharomyces cerevisiae
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DOI：
10.1074/jbc.m201667200
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发表时间：
2002-05-24
影响因子：
4.8
通讯作者：
Mizuta, K
中科院分区：
文献类型：
--
作者：
Miyoshi, K;Tsujii, R;Mizuta, K
分泌缺陷导致酵母核糖体蛋白和rRNA基因的转录抑制。为了阐明信号传导的机制，我们先前分离了不能响应分泌缺陷的rrs突变体，并且我们克隆了编码核糖体生物发生所需的核蛋白的RRS 1（Tsuno，A.，Miyoshi，K.，Tsujii河，Miyakawa，T.，和Mizuta，K（2000）Mol Cell Biol.20，2066-2074）。我们确定了重复的基因编码核糖体蛋白L11，RPL 11B作为野生型等位基因互补rrs 2突变，和RPL 11 A在双杂交筛选使用RRS 1作为诱饵。Rpl 11 p与Rrs 1 p共纯化后进行免疫沉淀分析。超电泳分析表明，Rrs 1 p与60 S前核糖体亚基紧密结合。这些结果表明，在响应分泌缺陷的信号需要60 S核糖体亚基，包括Rrs 1 p和Rpl 11 p的正常组装。
A secretory defect leads to transcriptional repression of both ribosomal protein and rRNA genes in yeast. To elucidate the mechanism of the signaling, we previously isolated rrs mutants that were unable to respond to a secretory defect, and we cloned RRS1 encoding a nuclear protein that was required for ribosome biogenesis (Tsuno, A., Miyoshi, K., Tsujii, R., Miyakawa, T., and Mizuta, K (2000) Mol Cell Biol. 20, 2066-2074). We identified duplicated genes encoding ribosomal protein L11, RPL11B as a wild-type allele complementing the rrs2 mutation, and RPL11A in two-hybrid screening using RRS1 as bait. Rpl11p was copurified with Rrs1p in immunoprecipitation analysis. Ultracentrifugation analysis revealed that Rrs1p associated fairly tightly with 60 S preribosomal subunits. These results suggest that signaling in response to a secretory defect requires the normal assembly of 60 S ribosomal subunits including Rrs1p and Rpl11p.