Expression and distribution of CB1 cannabinoid receptors in the central nervous system of the African cichlid fish Pelvicachromis pulcher
Expression and distribution of CB1 cannabinoid receptors in the central nervous system of the African cichlid fish Pelvicachromis pulcher
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DOI：
10.1002/cne.20502
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发表时间：
2005-05-16
影响因子：
2.5
通讯作者：
Franzoni, MF
中科院分区：
文献类型：
--
作者：
Cottone, E;Forno, S;Franzoni, MF
对低等脊椎动物（如硬骨鱼）大麻素系统的神经解剖学研究可能会提高我们对这种信号系统的生理作用的理解。在本研究中，CB1大麻素受体的表达已在硬骨鱼（慈鲷Pelvicachromis pulcher）的中枢神经系统中得到证实。此外，通过使用针对 CB1 受体氨基末端的纯化抗体，分析了 CB1 样免疫反应性。在端脑以及下丘脑的视前区和外侧漏斗叶中发现了免疫染色的神经元和静脉曲张。在垂体中观察到染色细胞。在顶盖前中央核和后结节中发现了几个含有强烈 CB1 样免疫反应性的细胞体和神经末梢，两者都位于间脑和中脑之间的过渡区域。在脑干中，CB1 免疫阳性比在前脑中受到更多限制，但背侧中脑被盖内的一些大的、强烈免疫阳性的神经细胞（可能是第三脑神经的运动神经元）除外。在小脑中，在大多数免疫阴性颗粒细胞中，其中一部分被免疫染色。还观察到一些阳性浦肯野细胞。在脊髓、腹侧灰质中，几个α运动神经元被染色。讨论了与其他脊椎动物中枢神经系统中 CB1 受体分布的相似性和差异，特别关注在包括前顶盖和肾小球核的区域中观察到的丰富的 CBI 免疫反应性，其特征是硬骨鱼中的特殊分化。 (c) 2005 年 Wiley-Liss, Inc.
Neuroanatomical investigation of the cannabinoid system in a lower vertebrate group such as teleost fishes might improve our understanding of the physiological role of such a signaling system. In the present study, the expression of a CB1 cannabinoid receptor has been demonstrated in the CNS of a teleost fish, the cichlid Pelvicachromis pulcher. Moreover, CB1-like immunoreactivity has been analyzed by using a purified antibody against the CB1 receptor amino-terminus. Immunostained neurons and varicosities were found through the telencephalon as well as in the preoptic area and lateral infundibular lobes of the hypothalamus. Stained cells were observed in the pituitary gland. Several cell bodies and nerve terminals containing an intense CB1-like immunoreactivity were found in the pretectal central nucleus and posterior tuberculum, both lying in a transitional region between diencephalon and mesencephalon. In the brainstem, the CB1 immunopositivity was more restricted than in the prosencephalon, with the exception of some large, intensely immunopositive nerve cells within the dorsal mesencephalic tegmentum, possibly motor neurons of the third cranial nerve. In the cerebellum, among a majority of immunonegative granule cells, a subset of them was immunostained. Some positive Purkinje cells were also observed. In the spinal cord, ventral gray matter, several alpha-motoneurons were stained. Similarities to and discrepancies from the CB1 receptor distributions in other vertebrate CNS are discussed, paying particular attention to the abundant CBI immunoreactivity observed in the area encompassing the pretectum and glomerular nucleus, which is characterized by a peculiar differentiation in bony fishes. (c) 2005 Wiley-Liss, Inc.