Human papillomavirus-specific serologic response in vulvar neoplasia.
Human papillomavirus-specific serologic response in vulvar neoplasia.
复制标题
外阴肿瘤中人乳头瘤病毒特异性血清学反应。
DOI：
10.1006/gyno.1996.0306
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Shah,KV
中科院分区：
文献类型：
--
作者：
Sun,Y;Hildesheim,A;Brinton,LA;Nasca,PC;Trimble,CL;Kurman,RJ;Viscidi,RP;Shah,KV
流行病学和病毒学证据表明，外阴浸润性鳞状细胞癌（SCC）具有病因异质性，基底细胞癌或疣状鳞状细胞癌（BWSCC）和外阴上皮内瘤变（VIN）与人乳头瘤病毒（HPV）感染有关，而角化性鳞状细胞癌（KSCC）是一种与HPV无关的恶性肿瘤。在本研究中，用ELISA检测了BWSCC、VIN和KSCC患者和对照组血清中hpv特异性抗体对HPV-16病毒样颗粒（VLP）的反应，并用放射免疫沉淀法检测了通过体外转录和翻译表达的HPV-16 E6和E7蛋白的抗体。hpv相关VIN（59.1%）和BWSCC（50.0%）中HPV-16 VLPs抗体的患病率明显高于KSCC（22.2%）和对照组（18.2%）。E6和E7蛋白抗体在BWSCC中比在其他任何组中更普遍。BWSCC（64.3%）和VIN（59.1%）的血清抗体阳性率明显高于KSCC（27.8%）和对照组（22.2%）。此外，在BWSCC和VIN病例中发现高抗体滴度的血清比对照组更频繁。这些数据提供了免疫学证据，支持VIN和BWSCC与HPV感染相关的观察，而不是KSCC。
Epidemiological and virological evidence suggests that invasive squamous cell carcinoma (SCC) of the vulva is etiologically heterogeneous and that basaloid or warty SCC (BWSCC) and vulvar intraepithelial neoplasia (VIN) are linked to human papillomavirus (HPV) infections while keratinizing SCC (KSCC) is a non-HPV-associated malignancy. In the present study, HPV-specific antibodies in sera of patients with BWSCC, VIN, and KSCC and of controls were examined by ELISA for antibodies reactive to HPV-16 virus-like particles (VLP) and in radioimmunoprecipitation assays for antibodies to HPV-16 E6 and E7 proteins expressed byin vitrotranscription and translation. The prevalences of antibodies to HPV-16 VLPs were significantly higher in HPV-associated VIN (59.1%) and BWSCC (50.0%) than in KSCC (22.2%) and controls (18.2%). Antibodies to E6 and E7 proteins were more prevalent in BWSCC than in any other groups. Prevalence of serum antibodies to any one of the antigen preparations was significantly higher in BWSCC (64.3%) and VIN (59.1%) than in KSCC (27.8%) and controls (22.2%). Also, sera with high antibody titers were found more frequently in BWSCC and VIN cases than in controls. These data provide immunological evidence in support of the observation that VIN and BWSCC, but not KSCC, are associated with HPV infections.