Allelotype analysis of intrahepatic cholangiocarcinoma
Allelotype analysis of intrahepatic cholangiocarcinoma
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DOI：
10.1038/modpathol.3880108
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发表时间：
2000-06-01
期刊：
影响因子：
7.5
通讯作者：
Kim, WH
中科院分区：
文献类型：
--
作者：
Kang, YK;Kim, YI;Kim, WH
为了确定肝内胆管细胞癌(ICC)的等位基因缺失的染色体位置，我们利用55个基因组范围的微卫星标记对36个ICC进行了等位基因研究。杂合性缺失以8p(65.6%)、17p(64.7%)和9p(64.5%)最常见，其次是18q(54.2%)、Ip(48.5%)、3p(44.8%)、9q(42.1%)、14q(41.7%)、6q(41.7%)和1q(40.6%)。等位基因丢失分数(FAL值)范围为0~0.731，平均0.322。分析FAL值与临床病理参数的关系发现，中低分化型ICC的FAL值明显高于高分化型ICC(P<；0.05)。总之，本研究首次定义了等位基因缺失的染色体总数以及可能参与ICC发生的染色体臂和/或区域。
To identify the chromosomal loci of allelic loss in intrahepatic cholangiocarcinoma (ICC), we performed an allelotype study of 36 ICCs using 55 genome-wide microsatellite markers. Loss of heterozygosity was found most frequently on 8p (65.6%), 17p (64.7%), and 9p (64.5%), followed by 18q (54.2%), Ip (48.5%), 3p (44.8%), 9q (42.1%), 14q (41.7%), 6q (41.7%), and 1q (40.6%). The fractional allelic loss (FAL) values ranged from 0 to 0.731 (mean, 0.322). Analysis of the relationship between FAL values and clinicopathologic parameters disclosed significantly higher FAL values in moderately to poorly differentiated ICCs than in well-differentiated ones (P < .05), In summary, this study defined for the first time the overall number of chromosomes having allelic loss and the chromosomal arms and/or regions potentially involved in the development of ICC.