Structural analyses of protoberberine alkaloids in medicine herbs by using ESI--FT-ICR-MS and HPLC-ESI-MSn
Structural analyses of protoberberine alkaloids in medicine herbs by using ESI--FT-ICR-MS and HPLC-ESI-MSn
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DOI：
10.1016/j.jpba.2004.11.026
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发表时间：
2005-03-09
影响因子：
3.4
通讯作者：
Liu, SY
中科院分区：
文献类型：
--
作者：
Wu, W;Song, FR;Liu, SY
采用高质量分辨率的持续非共振辐射（SORI）/碰撞诱导解离（CID）电喷雾电离傅里叶变换离子回旋共振质谱（ESI-FT-ICR-MS）研究了4种原小檗碱类生物碱的特征碎片离子。ESI-FT-ICR SORI-CID实验结果表明，在高质量分辨率下，可以得到明确的碎片离子组成，从而提出了原小檗碱生物碱的合理裂解途径。对原小檗碱类生物碱的CID和MSn特征碎片离子进行了讨论，这些碎片离子对原小檗碱类生物碱化合物的鉴定具有特异性和实用性。采用HPLC-ESI-MSn对四种中草药的提取物进行了分析。根据这些特征裂解途径、HPLC保留时间（t（R））和产物离子质谱，鉴定了6种原小檗碱生物碱的结构。本文采用选择离子监测法（SIM）对生物碱异构体进行了分离和鉴定。(c)2004 Elsevier B. V.保留所有权利。
The Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR-MS) using sustained off-resonance irradiation (SORI)/collision-induced dissociation (CID) method at high mass resolution has been first applied to investigate the characteristic fragment ions of four protoberberine alkaloids in medicine herbs. The ESI-FT-ICR SORI-CID experiment results demonstrate that the unambiguous elemental composition of fragment ions can be obtained at high mass resolution, then the logical fragmentation pathways of the protoberberine alkaloids has been proposed. The characteristic fragment ions of CID and MSn of protoberberine alkaloids have been discussed, which are specific and useful for the identification of some protoberberine alkaloid compounds. Then, the extracts of four kinds of medicine herbs have been analyzed by HPLC-ESI-MSn. According to these characteristic fragmentation pathways, the retention time (t(R)) of HPLC and mass spectra of product ion, the structures of six kinds of protoberberine alkaloids have been identified. And, in the present paper, the selected ion monitoring (SIM) method has been used to separate and identify the alkaloid isomers. (c) 2004 Elsevier B.V. All rights reserved.