Expression of myelin protein genes in quaking mouse brain.
Expression of myelin protein genes in quaking mouse brain.
复制标题
震颤小鼠大脑中髓磷脂蛋白基因的表达。
DOI：
10.1002/jnr.490200104
复制
发表时间：
1988
影响因子：
4.2
通讯作者：
Hogan,EL
中科院分区：
文献类型：
--
作者：
Konat,G;Trojanowska,M;Gantt,G;Hogan,EL
研究了髓鞘蛋白在主动髓鞘化震颤和对照脑中的表达。从18日龄和27日龄小鼠的大脑中提取RNA，并使用蛋白质脂质蛋白（PLP）、碱性蛋白（BP）和髓鞘相关糖蛋白（MAG）的cDNA探针进行northern blot分析。发现两个PLP转录本分别为3.2和2.4 kb（千碱基），而PB和MAG探针分别杂交到2.2和2.5 kb的单个区域。在18日龄或27日龄的震颤大脑中没有检测到转录模式的异常。在这9天的时间里，对照脑中的PLP和BP信息水平下降了约10%，而MAG信息水平下降了约50%。在18天大的震颤脑中，PLP和BP的表达严重降低，分别为对照值的三分之一和一半。27日龄时下降幅度较小。另一方面，在第18天和第27天，颤抖的大脑产生了更多的MAG mRNA，分别是对照组的1.6倍和3.2倍。结果表明，突变体PLP和BP基因表达减少，发育迟缓，而MAG基因表达增强，似乎逐渐失调。
The expression of myelin proteins in actively myelinating quaking and control brains was studied. RNA was extracted from the brains of 18‐ and 27‐day‐old mice and analyzed by northern blot using cDNA probes for proteolipid protein (PLP), basic protein (BP), and myelin‐associated glycoprotein (MAG). Two PLP transcripts of 3.2 and 2.4 kb (kilobase) were found, whereas PB and MAG probes hybridized to single regions of 2.2 and 2.5 kb, respectively. No abnormality in the transcript pattern was detectable in the quaking brain at either 18 or 27 days of age. Over this 9‐day period the level of PLP and BP message in the control brain decreased by approximately 10%, whereas the level of MAG message decreased by approximately 50%. In the 18‐day‐old quaking brain the expression of PLP and BP was severely reduced amounting to one‐third and one‐half of the control values, respectively. The reduction at the age of 27 days was less. On the other hand, the quaking brain produced more MAG mRNA amounting to 1.6‐ and 3.2‐fold control on the 18th and 27th day. The results indicate a reduced expression of the PLP and BP genes and a developmental delay in the mutant, whereas the genetic expression of MAG is enhanced and appears to be progressively dysregulated.