Effect of microbubble contrast agent during high intensity focused ultrasound ablation on rabbit liver in vivo
Effect of microbubble contrast agent during high intensity focused ultrasound ablation on rabbit liver in vivo
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DOI：
10.1016/j.ejrad.2011.06.002
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发表时间：
2012-04-01
影响因子：
3.3
通讯作者：
Hahn, Seong-Tae
中科院分区：
文献类型：
--
作者：
Chung, Dong Jin;Cho, Se Hyun;Hahn, Seong-Tae
目的：材料与方法：对16只活体兔肝进行高强度聚焦超声（HIFU）消融（强度为400 W/cm ~ 2，每次4s，共6次，每次间隔5s），在消融前和消融后静脉注射微泡造影剂（0.8ml）。使用Wilcoxon符号秩检验比较实时超声上组织消融所需的平均消融体积和时间。结果：微泡联合HIFU组的凝固性坏死体积明显大于单纯HIFU组（P < 0.05）。微泡联合HIFU组达到消融的时间明显短于单纯HIFU组（P < 0.05）。在组织消融形态分析中，单纯HIFU组的消融形态以金字塔形为主，微泡组和HIFU联合组的消融形态以金字塔形为主（P < 0.05）。凝固性坏死的模式分析后，在微泡和HIFU组合组中，10个样本中发现非空洞性坏死，6个样本中发现空洞性坏死。结论：与未使用微泡造影剂组相比，使用微泡造影剂组的HIFU消融范围更大，坏死组织更多。微泡造影剂可能通过增强HIFU的治疗效果而用于组织消融。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Objective: To evaluate the effect of a microbubble contrast agent (SonoVue) during HIFU ablation of a rabbit liver.Materials and methods: HIFU ablations (intensity of 400 W/ cm2 for 4 s, six times, with a 5 s interval between exposures) were performed upon 16 in vivo rabbit livers before and after intravenous injection of a microbubble contrast agent (0.8 ml). A Wilcoxon signed rank test was used to compare mean ablation volume and time required to tissue ablation on real-time US. Shape of ablation and pattern of coagulative necrosis were analyzed by Fisher's exact test.Results: The volume of coagulative necrosis was significantly larger in the combination microbubble and HIFU group than in the HIFU alone group (P < 0.05). Also, time to reach ablation was shorter in the combination microbubble and HIFU group than in the HIFU alone group (P < 0.05). When analyzing the shape of tissue ablation, a pyramidal shape was more prevalently in the HIFU alone group compared to the combination microbubble and HIFU group (P < 0.05). Following an analysis of the pattern of coagulative necrosis, non-cavitary necrosis was found in ten and cavitary necrosis in six of the samples in the combination microbubble and HIFU group. Conversely, non-cavitary necrosis occurred in all 16 samples in the HIFU alone group (P < 0.05).Conclusion: HIFU of in vivo rabbit livers with a microbubble contrast agent produced larger zones of ablation and more cavitary tissue necrosis than without the use of a microbubble contrast agent. Microbubble contrast agents may be useful in tissue ablation by enhancing the treatment effect of HIFU. (C) 2011 Elsevier Ireland Ltd. All rights reserved.