New compatible solutes related to di-myo-inositol-phosphate in members of the order Thermotogales
New compatible solutes related to di-myo-inositol-phosphate in members of the order Thermotogales
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DOI：
10.1128/jb.178.19.5644-5651.1996
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发表时间：
1996-10-01
影响因子：
3.2
通讯作者：
Santos, H
中科院分区：
文献类型：
--
作者：
Martins, LO;Carreto, LS;Santos, H
本文用核磁共振波谱法研究了六种嗜热袍菌胞内有机溶质的积累。在海栖热袍菌和那不勒斯栖热袍菌中鉴定出新发现的化合物二-2-O-β-甘露糖基-二-肌醇-1，1 '（3，3'）-磷酸和二-肌醇-1，3 '-磷酸。在最适温度和盐度下，有机溶质库除了二-肌醇-1，3 ′-磷酸和二-2-O-β-甘露糖基-二-肌醇-1，1 ′（3，3 ′）-磷酸之外，还由二-肌醇-1，1 ′（3，3 '）-磷酸盐。后两种溶质的浓度在超最适生长温度下急剧增加，而β-谷氨酸主要响应于盐胁迫而增加。然而，二-肌-肌醇-1，1 '（3，3'）-磷酸盐是在高于最适生长温度的盐度下的主要相容性溶质。海藻糖和甜菜碱。在最佳生长条件下，在Thermotoga thermarum和Fervidobacterium islandicum中没有检测到有机溶质，它们具有低盐要求或没有。
The accumulation of intracellular organic solutes was examined in six species of the order Thermotogales by nuclear magnetic resonance spectroscopy, The newly discovered compounds di-2-O-beta-mannosyl-di-myo-inositol-1,1'(3,3')-phosphate and di-myo-inositol-1,3'-phosphate were identified in Thermotoga maritima and Thermotoga neapolitana, In the latter species, at the optimum temperature and salinity the organic solute pool was composed of di-myo-inositol-1,1'(3,3')-phosphate, beta-glutamate, and alpha-glutamate in addition to di-myo-inositol-1,3'-phosphate and di-2-O-beta-mannosyl-di-myo-inositol-1,1'(3,3')-phosphate. The concentrations of the last two solutes increased dramatically at supraoptimal growth temperatures, whereas beta-glutamate increased mainly in response to a salinity stress, Nevertheless, di-myo-inositol-1,1'(3,3')-phosphate was the major compatible solute at salinities above the optimum for growth, The amino acids alpha-glutamate and proline were identified under optimum growth conditions in Thermosipho africanus, and beta-mannosylglycerate, trehalose, and glycine betaine were detected in Petrotoga miotherma. Organic solutes were not detected, under optimum growth conditions, in Thermotoga thermarum and Fervidobacterium islandicum, which have a low salt requirement or none.