The decomposition of cellulose by aerobic bacteria
The decomposition of cellulose by aerobic bacteria
复制标题
DOI：
10.1128/jb.15.4.223-234.1928
复制
发表时间：
1928-04-01
影响因子：
3.2
通讯作者：
Dubos, RJ
中科院分区：
文献类型：
--
作者：
Dubos, RJ
结果表明，通过使用这种简单且特定的培养基：NaNO3，0.50 gm.，需氧纤维素分解细菌可以快速且大量生长。 K2HPO4，1.0 克； MgSO4·7H2O，0.50克； KC1，0.50 克； FeSO4-7 H2O，0.01 克；蒸馏水，1000 克。五cc。将部分该介质引入装有部分浸没的滤纸条的试管中。弱碱性反应（pH 7.5）有利于细菌生长，但会阻碍真菌生长。低浓度的 N 盐缩短了孵育时间，因为在几乎所有情况下，36-72 小时后即可记录纤维素分解。 28°C。即使使用1个或很少的细胞进行接种也能获得生长。通过这种方法分离了属于 3 个生理组群的生物体的纯培养物： (a) 严格需氧生物[长划线]不能利用除纤维素之外的任何测试的碳化合物； (b) 严格需氧菌[长划线]纤维素分解菌在淀粉琼脂上生长良好，但在营养琼脂上生长不良； (c) 兼性厌氧菌[长划线]纤维素分解者，在所有普通培养基中生长良好。
It is shown that a rapid and abundant growth of aerobic, cellulose-decomposing bacteria is obtained by use of this simple and specific medium: NaNO3, 0.50 gm.; K2HPO4, 1.0 gm.; MgSO4-7 H2O, 0.50 gm.; KC1, 0.50 gm.; FeSO4-7 H2O, 0.01 gm.; distilled H20,1000 gm. Five cc. portions of this medium are introduced into test tubes containing a strip of filter paper partly immersed. The slightly alkaline reaction (pH 7.5) favors bacterial but retards fungous growth. The low concentration of N salts shortens the incubation period, since, in almost all cases, cellulose decomposition can be recorded after 36-72 hrs. at 28[degree]C. Growth is obtained even when 1 or few cells are used for inoculation. Pure cultures of organisms belonging to 3 physiological groups have been isolated by this method: (a) Strict aerobes[long dash]unable to utilize any of the carbon compounds tested, except cellulose; (b) strict aerobes[long dash]cellulose decomposers growing well on starch agar but not on nutrient agar; (c) facultative anaerobes[long dash]cellulose decomposers, growing well in all ordinary media.