Pancreatic intraepithelial neoplasia and ductal adenocarcinoma induced by DMBA in mice
Pancreatic intraepithelial neoplasia and ductal adenocarcinoma induced by DMBA in mice
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DOI：
10.1016/j.surg.2006.02.012
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发表时间：
2006-11-01
期刊：
影响因子：
3.8
通讯作者：
Rohde, Luiz
中科院分区：
文献类型：
--
作者：
Osvaldt, Alessandro B.;Wendt, Luiz R.;Rohde, Luiz
背景资料。胰腺导管腺癌远期预后较差。不仅要了解胰腺癌的生物学行为，而且要了解胰腺上皮内瘤变(Panin)的早期胰腺病变，并开发新的治疗方法，实验模型是必要的。本研究的目的是根据Panin分类系统评价7，12-二甲基-1，2-苯并茂(DMBA)对小鼠胰腺的致癌作用。90只雄性、肌肉小鼠、CF-1小鼠接受了正中剖腹手术，并将1毫克DMBA植入胰腺近端，用荷包缝合固定。30天和60天后处死小鼠，取出的胰腺用福尔马林固定，石蜡包埋，苏木精-伊红染色进行组织学分析。这些标本。由2位病理医生盲法评估是否存在下列组织学改变：正常导管、反应性增生、Panin-1A、Panin-1B、Panin-2和Panin-3，以及腺癌。在30天组中，病理评估显示4例(17%)反应性增生，16例(67%)PAN皮损，4例(17%)腺癌。在60d组中，有10例(27%)有反应性增生，13例(35%)有Panin皮损，14例(38%)有腺癌。组间比较差异有统计学意义(P
Background. Pancreatic ductal adenocarcinoma has a poor long-term prognosis. Experimental models are necessary to understand not only its biologic behavior, but also the early pancreatic lesions known as pancreatic intraepithelial neoplasia (PanIN) and to develop new treatments. The aim of this study was to evaluate pancreatic carcinogenesis induced by 7,12-dimethyl-1, 2-benzanthracene (DMBA) implantation in mice according to the PanIN classification system.Methods. Ninety male, Mus musculus, CF-1 mice underwent a median laparotomy and 1 mg of DMBA was implanted into the proximal pancreas held in place by a purse-string suture. Mice were killed after 30 and 60 days after which the excised pancreata were fixed in formalin, embedded in paraffin, and stained with hematoxylin-eosin for histologic analysis. The specimens. were evaluated blind by 2 pathologists for the presence of the following histology: normal ducts, reactive hyperplasia, PanIN-1A, PanIN-1B, PanIN-2, and PanIN-3, and adenocarcinoma.Results. In the 30-day group, pathologic evaluation showed, 4 (17%) reactive hyperplasia, 16 (67%) PanIN lesions, and.4 (17%) adenocarcinomas. In the 60-day group, there were 10 (27%) specimens with reactive hyperplasia, 13 (35%) with PanIN lesions, and 14 (38%) with adenocarcinomas. The difference between groups was statistically significant (P