Introduction of a Foreign Gene into Zebrafish and Medaka Cells Using Adenoviral Vectors
Introduction of a Foreign Gene into Zebrafish and Medaka Cells Using Adenoviral Vectors
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DOI：
10.1089/zeb.2009.0596
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发表时间：
2009-09-01
期刊：
影响因子：
2
通讯作者：
Sakai, Noriyoshi
中科院分区：
文献类型：
--
作者：
Kawasaki, Toshihiro;Saito, Kenji;Sakai, Noriyoshi
病毒载体代表了一种易于处理的系统，可以有效地将外源基因导入不同的靶细胞，因此是一种潜在的强大的遗传操作工具。在我们目前的研究中，我们研究了哺乳动物病毒载体，腺病毒（Ads），腺相关病毒，慢病毒的感染效率的支持细胞系和新建立的细胞系从一个单一的胚胎在斑马鱼。在所测试的病毒载体中，Ads在斑马鱼细胞中显示出10（7）-10（8）绿色荧光蛋白转导单位（gtu）/mL的最高感染效率。另外，腺病毒载体也以10（5）gtu/mL感染青鳉睾丸体细胞系，所述青鳉睾丸体细胞系由p53缺陷突变体的睾丸建立。此外，我们发现Ads可以成功感染培养的雄性斑马鱼生殖细胞。因此，我们的研究结果表明，腺病毒载体可以作为一个染色体非整合载体系统在斑马鱼。
Viral vectors represent a tractable system that can efficiently introduce an exogenous gene into different target cells and are thus a potentially powerful genetic manipulation tool. In our current study, we investigated the infection efficiency of mammalian virus vectors, adenoviruses ( Ads), adeno-associated viruses, and lentiviruses to the Sertoli cell line and the newly established cell line from a single embryo in zebrafish. Among the viral vectors tested, Ads showed the highest infection efficiency of 10(7)-10(8) green fluorescent protein-transducing units (gtu)/mL in zebrafish cells. In addition, the adenoviral vector was also infected at 10(5) gtu/mL in the medaka testicular somatic cell line that was established from the testes of p53-deficient mutant. Further, we found that Ads could successfully infect cultured male zebrafish germ cells. Our results thus indicate that the adenoviral vector could be used as a chromosomally nonintegrating vector system in zebrafish.