Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells.
Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells.
复制标题
斑马鱼神经丘传入神经元的方向选择性受突触前毛细胞中的 Emx2 调节
DOI：
10.7554/elife.35796
复制
发表时间：
2018-04-19
期刊：
影响因子：
7.7
通讯作者：
Kindt KS
中科院分区：
文献类型：
--
作者：
Ji YR;Warrier S;Jiang T;Wu DK;Kindt KS
在侧线系统的神经肥大中，感觉毛细胞(HC)顶部的毛束的方向使鱼能够探测到水流的方向。每个神经肥大显示出沿两个相反方向排列的毛束，每个传入神经元只支配相同方向的毛细胞。此前，我们表明这种对抗是通过Emx2在一半的HCS中表达来建立的，在那里它介导了发束逆转(酱等人，2017)。在这里，我们表明Emx2也调节神经元的选择：传入神经元支配Emx2阳性或阴性的HC。在emx2基因敲除和功能获得的神经肥大细胞中，所有的HC都是单向的，传入神经元的神经模式和生理反应取决于Emx2的存在或不存在。我们的结果表明，Emx2通过两个不同的过程来调节神经肥大细胞的方向选择性：调节毛细胞的毛束方向和选择传入神经元靶点。
The orientation of hair bundles on top of sensory hair cells (HCs) in neuromasts of the lateral line system allows fish to detect direction of water flow. Each neuromast shows hair bundles arranged in two opposing directions and each afferent neuron innervates only HCs of the same orientation. Previously, we showed that this opposition is established by expression of Emx2 in half of the HCs, where it mediates hair bundle reversal (Jiang et al., 2017). Here, we show that Emx2 also regulates neuronal selection: afferent neurons innervate either Emx2-positive or negative HCs. In emx2 knockout and gain-of-function neuromasts, all HCs are unidirectional and the innervation patterns and physiological responses of the afferent neurons are dependent on the presence or absence of Emx2. Our results indicate that Emx2 mediates the directional selectivity of neuromasts by two distinct processes: regulating hair bundle orientation in HCs and selecting afferent neuronal targets.