Long-term monitoring of the human intestinal microbiota composition
Long-term monitoring of the human intestinal microbiota composition
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DOI：
10.1111/1462-2920.12023
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发表时间：
2013-04-01
影响因子：
5.1
通讯作者：
de Vos, Willem M.
中科院分区：
文献类型：
--
作者：
Rajilic-Stojanovic, Mirjana;Heilig, Hans G. H. J.;de Vos, Willem M.
定植于人类肠道的微生物群是复杂的，其结构对我们每个人来说都是特定的。在这项研究中，我们扩展了关于特定对象微生物区系稳定性的知识，并表明该生态系统在短期间隔(10年)内是稳定的。使用一种全面的、重复性高的系统发育微阵列HITChip分析了五名无关的健康受试者的粪便微生物区系组成。结果表明，抗生素的使用、特定饮食方案的应用和长途旅行对微生物区系组成的影响有限。几个厌氧菌属，包括双歧杆菌和拟杆菌属和菲尔米丘斯门中的一些属，表现出比总微生物区系明显更高的相似性。虽然肠道微生物区系包含特定于对象的物种，这些物种的存在在多年中得到保存，但它们的相对丰度变化很大。因此，最近提出的肠型状态似乎是微生物区系的一个不同特征。我们的数据表明，肠道微生物群包含一个永久定居者的核心群落，在整个成年期，环境引入的微生物群的变化主要影响特定微生物物种的丰度，但不影响特定微生物物种的存在。
The microbiota that colonizes the human intestinal tract is complex and its structure is specific for each of us. In this study we expand the knowledge about the stability of the subject-specific microbiota and show that this ecosystem is stable in short-term intervals (10 years). The faecal microbiota composition of five unrelated and healthy subjects was analysed using a comprehensive and highly reproducible phylogenetic microarray, the HITChip. The results show that the use of antibiotics, application of specific dietary regimes and distant travelling have limited impact on the microbiota composition. Several anaerobic genera, including Bifidobacterium and a number of genera within the Bacteroidetes and the Firmicutes phylum, exhibit significantly higher similarity than the total microbiota. Although the gut microbiota contains subject-specific species, the presence of which is preserved throughout the years, their relative abundance changes considerably. Consequently, the recently proposed enterotype status appears to be a varying characteristic of the microbiota. Our data show that the intestinal microbiota contains a core community of permanent colonizers, and that environmentally introduced changes of the microbiota throughout adulthood are primarily affecting the abundance but not the presence of specific microbial species.