Copper homeostasis in Salmonella is atypical and copper-CueP is a major periplasmic metal complex.
Copper homeostasis in Salmonella is atypical and copper-CueP is a major periplasmic metal complex.
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DOI：
10.1074/jbc.m110.145953
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发表时间：
2010-08-13
期刊：
影响因子：
--
通讯作者：
Cavet JS
中科院分区：
文献类型：
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作者：
Osman D;Waldron KJ;Denton H;Taylor CM;Grant AJ;Mastroeni P;Robinson NJ;Cavet JS
肠道沙门氏菌。鼠伤寒杆菌（肠炎沙门氏菌）；鼠伤寒杆菌有两种金属运输p1型atp酶，它们的作用在铜含量升高的情况下很大程度上重叠。与野生型或单个突变体相比，缺乏两种atp酶的突变体铜积累过多。这种atp酶的重复在细菌的铜耐受性中是不寻常的。这两种atp酶都受MerR家族金属响应转录激活因子的控制。质周铜配合物分析表明铜- cuep是主要的金属池之一。cueP的表达最近被证明是由与p1型atp酶基因（copA）相同的金属反应激活因子控制的，铜- cueP是肠球菌铜稳态的另一个非典型特征。沙门氏菌感染。在野生型肠链球菌中，由copA启动子驱动的报告结构检测到升高的铜。巨噬细胞感染期间的鼠伤寒。缺失两种atp酶的双突变体在培养的巨噬细胞内也显示出存活率降低。据推测，巨噬细胞内铜含量升高可能选择了致病微生物（如肠球菌）的特异性铜抗性系统。沙门氏菌感染。
Salmonella enterica sv. typhimurium (S. enterica sv. Typhimurium) has two metal-transporting P1-type ATPases whose actions largely overlap with respect to growth in elevated copper. Mutants lacking both ATPases over-accumulate copper relative to wild-type or either single mutant. Such duplication of ATPases is unusual in bacterial copper tolerance. Both ATPases are under the control of MerR family metal-responsive transcriptional activators. Analyses of periplasmic copper complexes identified copper-CueP as one of the predominant metal pools. Expression of cueP was recently shown to be controlled by the same metal-responsive activator as one of the P1-type ATPase genes (copA), and copper-CueP is a further atypical feature of copper homeostasis in S. enterica sv. Typhimurium. Elevated copper is detected by a reporter construct driven by the promoter of copA in wild-type S. enterica sv. Typhimurium during infection of macrophages. Double mutants missing both ATPases also show reduced survival inside cultured macrophages. It is hypothesized that elevated copper within macrophages may have selected for specialized copper-resistance systems in pathogenic microorganism such as S. enterica sv. Typhimurium.