Protein kinase C-mediated intracellular alkalinization in rat and rabbit aortic smooth muscle cells.
Protein kinase C-mediated intracellular alkalinization in rat and rabbit aortic smooth muscle cells.
复制标题
蛋白激酶 C 介导大鼠和兔主动脉平滑肌细胞的细胞内碱化。
DOI：
10.1016/0014-2999(87)90574-7
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发表时间：
1987
影响因子：
5
通讯作者：
Deth,RC
中科院分区：
文献类型：
--
作者：
Danthuluri,NR;Berk,BC;Brock,TA;CragoeJr,EJ;Deth,RC
通过使用 pH 敏感荧光染料 2,7-双-羧乙基-5,6-羧基荧光素 (BCECF)，研究了蛋白激酶 C (C-激酶) 激活对培养的大鼠 (RASM) 和兔 (RBASM) 主动脉平滑肌细胞的细胞内 pH (pHi) 的影响。已知的 C 激酶激活剂 12-O-十四烷酰佛波醇-13-乙酸酯 (TPA)、佛波醇 12,13-二丁酸酯 (PDBu) 和 mezerine 以及激动剂血管紧张素 II 均会导致在无血清条件下生长的 RASM 和 RBASM 细胞的细胞内碱化约 0.1–0.15 pH 单位。 TPA 诱导的碱化对 Na+/H+ 交换阻滞剂阿米洛利和 5-N-乙基异丙基阿米洛利 (EIPA) 敏感。这些结果表明，蛋白激酶 C 激活导致血管平滑肌细胞胞内碱化，pH 值的增加可能在受体偶联动脉收缩中发挥重要作用。
The influence of protein kinase C (C-kinase) activation on intracellular pH (pHi) of cultured rat (RASM) and rabbit (RBASM) aortic smooth muscle cells was studied by employing a pH-sensitive fluorescent-dye 2,7-bis-carboxyethyl-5,6-carboxyfluorescein (BCECF). The known C-kinase activators 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol 12,13-dibutyrate (PDBu) and mezerine as well as the agonist angiotensin II each caused an intracellular alkalinization of approximately 0.1–0.15 pH units in RASM and RBASM cells grown in serum-free conditions. TPA-induced alkalinization was sensitive to the Na+/H+exchange blockers amiloride and 5-N-ethylisopropyl-amiloride (EIPA). These results suggest that protein kinase C activation leads to intracellular alkalinization in vascular smooth muscle cells and the increase in pHimight play an important role in receptor-coupled arterial contraction.