Genetic diversity, population structure and association analysis in cut chrysanthemum (Chrysanthemum morifolium Ramat.)
Genetic diversity, population structure and association analysis in cut chrysanthemum (Chrysanthemum morifolium Ramat.)
复制标题
切花菊（Chrysanthemum morifolium Ramat.）遗传多样性、种群结构及关联分析
DOI：
10.1007/s00438-016-1166-3
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发表时间：
2016-06-01
影响因子：
3.1
通讯作者：
Chen, Fadi
中科院分区：
文献类型：
--
作者：
Li, Pirui;Zhang, Fei;Chen, Fadi
对植物种质资源的遗传多样性进行研究有助于种质资源的有效管理和遗传改良。切花菊（Chrysanthemum morifolium Ramat.）是重要的经济观赏树种。随着种质资源的不断交流和育种活动的不断深入，它仍然是遗传学研究的一个重要课题。本研究的目的是表征全球收集的159个品种的遗传多样性和群体结构，并应用关联作图的方法来确定基于DNA的标记与5个植物构型性状和6个花序性状。基因分型表明，有没有缺乏遗传多样性的集合，成对的亲属关系值相对较低。基于种群结构的贝叶斯模型的聚类并没有反映种源或花序类型的已知变异。主坐标分析，但是，能够区分大多数品种根据这两个标准。每100个标记对中约有1个表现出一定程度的连锁不平衡。关联分析确定了许多与一个或多个性状连锁的标记。其中一些协会在两个赛季中表现强劲。这些发现提供了对切花菊品种遗传多样性和种群结构的深入了解，并深入了解了植物结构和花序相关性状的遗传控制。
Characterizing the genetic diversity present in a working set of plant germplasm can contribute to its effective management and genetic improvement. The cut flower chrysanthemum (Chrysanthemum morifolium Ramat.) is an economically important ornamental species. With the repeated germplasm exchange and intensive breeding activities, it remains a major task in genetic research. The purpose of the present study was to characterize the genetic diversity and the population structure of a worldwide collection of 159 varieties, and to apply an association mapping approach to identify DNA-based markers linked to five plant architecture traits and six inflorescence traits. The genotyping demonstrated that there was no lack of genetic diversity in the collection and that pair-wise kinship values were relatively low. The clustering based on a Bayesian model of population structure did not reflect known variation in either provenance or inflorescence type. A principal coordinate analysis was, however, able to discriminate most of the varieties according to both of these criteria. About 1 in 100 marker pairs exhibited a degree of linkage disequilibrium. The association analysis identified a number of markers putatively linked to one or more of the traits. Some of these associations were robust over two seasons. The findings provide an in-depth understanding of genetic diversity and population structure present in cut flower chrysanthemum varieties, and an insight into the genetic control of plant architecture and inflorescence-related traits.