Electrochemical characteristics of a gold nanoparticle-modified controlled enzyme?electrode contact junction electrode
Electrochemical characteristics of a gold nanoparticle-modified controlled enzyme?electrode contact junction electrode
复制标题
金纳米粒子修饰的受控酶电极接触连接电极的电化学特性
DOI：
10.1007/s10529-021-03092-3
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发表时间：
2021
影响因子：
2.7
通讯作者：
Suye Shin-ichiro
中科院分区：
文献类型：
--
作者：
Mori Saki;Kitta Yohei;Sakamoto Hiroaki;Takamura Eiichiro;Suye Shin-ichiro
在电极材料的表面上固定有酶、抗体等生物分子的生物装置能够将化学能转换为电能，特别是作为生物电池、生物传感器，有望对解决能源问题、开发医疗测量等做出贡献。器件性能取决于生物分子层和电极材料之间形成的界面，并且该界面需要同时实现高效的酶促反应和电子转移。然而，当酶被固定在材料表面上时，由于酶和电极表面之间的相互作用，酶经历结构变化，使得难以使材料表面上的酶分子的功能最大化。在本研究中，我们假设，通过使酶与电极之间的接触面积极小并将其吸附为点，可以减少酶的结构变化并提高电化学性能。因此，我们的目标是开发一种高功率的生物器件，通过在酶和电极之间插入金纳米颗粒（AuNPs）来保持酶的结构和活性。研究了吡咯并喹啉醌依赖的葡萄糖脱氢酶在5-40 nm金纳米粒子上的催化和电化学性质。我们发现，不同的颗粒之间的特性，和酶吸附在20 nm的金纳米粒子表现出最好的电化学特性。
Biodevices in which biomolecules such as enzymes and antibodies are immobilized on the surface of electrode materials are capable of converting chemical energy into electrical energy, and are expected to contribute to solving energy problems and developing medical measurements especially as biobatteries and biosensors. Device performance depends on the interface formed between the biomolecule layer and electrode material, and the interface is required to simultaneously achieve a highly efficient enzymatic reaction and electron transfer. However, when enzymes were immobilized on a material surface, the enzymes undergoes a structural change due to the interaction between the enzyme and the electrode surface, making it difficult to maximize the function of the enzyme molecule on the material surface. In this study, we postulate that the structural change of the enzyme would be reduced and the electrochemical performance improved by making the contact area between the enzyme and the electrode extremely small and adsorbing it as a point. Therefore, we aimed to develop a high-power biodevice that retains enzyme structure and activity by interposing gold nanoparticles (AuNPs) between the enzyme and the electrode. The enzymatic and electrochemical properties of pyrroloquinoline quinone-dependent glucose dehydrogenase adsorbed on AuNPs of 5–40 nm diameter were investigated. We found that the characteristics differed among the particles, and the enzyme adsorbed on 20 nm AuNPs showed the best electrochemical characteristics.