Electroacupuncture Pretreatment Attenuates Intestinal Injury after Autogenous Orthotopic Liver Transplantation in Rats via the JAK/STAT Pathway
Electroacupuncture Pretreatment Attenuates Intestinal Injury after Autogenous Orthotopic Liver Transplantation in Rats via the JAK/STAT Pathway
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DOI：
10.1155/2020/9187406
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发表时间：
2020-08-04
影响因子：
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通讯作者：
Weng，Yiqi
中科院分区：
文献类型：
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作者：
Jia，Lili;Yu，Wenli;Weng，Yiqi
背景肝移植会导致自身损伤并影响远端器官，如肺、肾和肠。术后肠功能障碍与住院时间延长有关，并影响患者的健康和生活质量。电针具有多器官保护作用。然而，潜在的机制，EA对肝移植后肠损伤的保护作用仍不清楚。方法：建立自体原位肝移植（AOLT）模型后，我们研究了EA预处理对AOLT后肠损伤的影响。我们使用JAK 2特异性抑制剂AG 490来探索潜在的机制。组织学分析和细胞凋亡测定用于评估肠损伤。AOLT后测定氧化应激指数和炎症反应。Western blot检测JAK 2、STAT 1和STAT 3的磷酸化水平。HE染色和TUNEL法显示，电针预处理可减轻AOLT后肠组织的损伤。电针预处理可抑制AOLT后肠组织中p-JAK 2、p-STAT 1和p-STAT 3的表达。经JAK 2特异性抑制剂AG 490治疗后，肠道损伤得到平衡。提示电针预处理可通过抑制JAK/STAT信号通路减轻AOLT后肠损伤。这些结果为电针的潜在疗效提供了基础证据。
Background. Liver transplantation induces self‐injury and affects remote organs, such as the lung, kidney, and intestine. Postoperative intestinal dysfunction has been associated with prolonged hospitalization and affects a patient’s health and quality of life. Electroacupuncture (EA) has been proven effective in multiple organ protection. However, the potential mechanism underlying the protective effects of EA on intestinal injury after liver transplantation remains unclear.Methods.After establishing an autogenous orthotopic liver transplantation (AOLT) model, we studied the effects of EA pretreatment on intestinal injury after AOLT. We used the JAK2‐specific inhibitor AG490 to explore the underlying mechanism. Histological analysis and apoptosis assays were used to evaluate intestinal injury. Oxidative stress index and inflammatory response were also measured after AOLT. Furthermore, we detected the phosphorylation levels of JAK2, STAT1, and STAT3 by Western blot.Results. We found that pretreatment with EA alleviated intestinal injury after AOLT, as shown by HE staining and TUNEL methods. EA pretreatment inhibited the expressions of p‐JAK2, p‐STAT1, and p‐STAT3 in the intestines after AOLT. Upon treatment with JAK2‐specific inhibitor AG490, intestinal injury was balanced.Conclusion. The data indicated EA pretreatment alleviated intestinal injury after AOLT by inhibiting the JAK/STAT signaling pathway. These results provide basic evidence to support the potential therapeutic efficacy of EA.