Immunological properties of the primer-independent glucosyltransferase of Streptococcus mutans serotypes d and g.
Immunological properties of the primer-independent glucosyltransferase of Streptococcus mutans serotypes d and g.
复制标题
变形链球菌血清型 d 和 g 的引物非依赖性葡萄糖基转移酶的免疫学特性。
DOI：
10.1099/00221287-134-5-1223
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Takehara
中科院分区：
文献类型：
--
作者：
Y. Yamashita;T. Shigeoka;N. Hanada;T. Takehara
变形链球菌g血清型分泌至少三种具有不同酶学和免疫学特性的葡萄糖基转移酶。其中一个是引物非依赖性酶，似乎是其他两个引物依赖性酶的引物来源。最近，我们从S.变异株AHT-k血清型g.在本研究中，我们检测了针对引物非依赖性葡糖基转移酶的抗血清的特异性，使用多种血清型的S.变种人该抗血清与血清型g和血清型d、a菌株的细胞外培养液有交叉反应，而与其他血清型菌株无交叉反应，表明该菌株分泌不依赖引物的葡萄糖基转移酶。sobrinus和S. cricetus，而不是S.和S.老鼠抗血清不能完全抑制酶的活性，即使在超过两倍的抗体过量时，通过用固定化葡萄球菌蛋白A间接沉淀测定。
Streptococcus mutans serotype g secretes at least three kinds of glucosyltransferase with different enzymological and immunological properties. One of them is a primer-independent enzyme and seems to be the source of primer for the others, both of which are primer-dependent enzymes. Recently, we purified the primer-independent enzyme, the third glucosyltransferase in this group from S. mutans strain AHT-k serotype g. In the present study, we examined the specificity of the antiserum against the primer-independent glucosyltransferase using extracellular culture-conditioned fluids of many strains of the various serotypes of S. mutans. The antiserum cross-reacted with the extracellular culture fluids from strains of serotypes d and a, in addition to serotype g, but not with those of other serotypes, indicating that the primer-independent glucosyltransferase is secreted by the S. sobrinus and S. cricetus, but not by S. mutans and S. rattus. The antiserum did not completely inhibit the activity of the enzyme, even at more than twofold antibody excess, determined by indirect precipitation with immobilized staphylococcal protein A.