Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts
Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts
复制标题
DOI：
10.1016/j.febslet.2014.09.031
复制
发表时间：
2014-11-03
期刊：
影响因子：
3.5
通讯作者：
Sugita, Mamoru
中科院分区：
文献类型：
--
作者：
Ichinose, Mizuho;Uchida, Masato;Sugita, Mamoru
小立碗藓叶绿体中有两个RNA编辑位点。在这里，我们确定了一个新的DYW亚类五肽重复序列（PPR）蛋白，PpPPR_45，作为叶绿体RNA编辑因子在P. patens。PpPPR_45基因的敲低降低了叶绿体rps 14-C2位点的RNA编辑程度，而PpPPR_45的过表达增加了rps 14-C2位点及其邻近C位点的RNA编辑水平。这表明PpPPR_45的表达水平影响两个相邻位点的RNA编辑程度。(C)2014年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The moss Physcomitrella patens has two RNA editing sites in the chloroplasts. Here we identified a novel DYW-subclass pentatricopeptide repeat (PPR) protein, PpPPR_45, as a chloroplast RNA editing factor in P. patens. Knockdown of the PpPPR_45 gene reduced the extent of RNA editing at the chloroplast rps14-C2 site, whereas over-expression of PpPPR_45 increased the levels of RNA editing at both the rps14-C2 site and its neighboring C site. This indicates that the expression level of PpPPR_45 affects the extent of RNA editing at the two neighboring sites. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.