Quantification of Amino Acids in a Single Cell by Microchip Electrophoresis with Chemiluminescence Detection
Quantification of Amino Acids in a Single Cell by Microchip Electrophoresis with Chemiluminescence Detection
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DOI：
10.1007/978-1-61779-445-2_28
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Zhao, Shulin
中科院分区：
文献类型：
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作者：
Liu, Yi-Ming;Zhao, Shulin
分析单个细胞可以检测大量正常细胞中存在的少数异常细胞。这种能力对于了解疾病，如癌症和糖尿病至关重要。微芯片电泳（MCE）是单细胞分析的首选技术。然而，由于微流体装置中的通道非常小，因此在微流体平台上实现期望的测定灵敏度仍然是一个挑战。在这里，我们描述了MCE方法与高灵敏度的化学发光检测同时测定存在于单细胞中的多种氨基酸。
Analyzing individual cells allows detecting a minor group of abnormal cells present in a large population of normal cells. This ability can be essential to understanding diseases, such as cancer and diabetes. Microchip electrophoresis (MCE) is the technique of choice for single-cell analysis. However, since the channels in microfluidic devices are very small, achieving the desired assay sensitivity on a microfluidic platform remains a challenge. Here, we describe an MCE method with highly sensitive chemiluminescence detection for simultaneous determination of multiple amino acids present in single cells.