PREPARATION AND CHARACTERIZATION OF BONE AND TOOTH COLLAGEN FOR ISOTOPIC ANALYSIS
PREPARATION AND CHARACTERIZATION OF BONE AND TOOTH COLLAGEN FOR ISOTOPIC ANALYSIS
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DOI：
10.1016/0305-4403(90)90007-r
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发表时间：
1990-07-01
影响因子：
2.8
通讯作者：
AMBROSE, SH
中科院分区：
文献类型：
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作者：
AMBROSE, SH
提出了一种广泛使用的方法制备的骨和牙齿胶原蛋白的碳和氮同位素比值的成岩变化的识别标准。本文描述了359名历史和史前非洲人以及现代和史前东非非人类哺乳动物的牙齿和骨骼中胶原蛋白浓度、原子C：N比以及胶原蛋白中碳和氮浓度的测量结果。还提供了四种骨胶原脂质的碳同位素比值。 与骨相比，整个牙齿具有显著较低的胶原蛋白浓度，胶原蛋白中较低的碳和氮浓度，以及相似的C：N比。碳和氮浓度以及C：N比在广泛的胶原蛋白浓度范围内相对恒定。然而，具有非常低的胶原蛋白浓度的史前标本具有高度可变的C：N比率，胶原蛋白中的碳和氮浓度非常低，并且与胶原蛋白不同，碳和氮同位素比率稳定。 在从保存良好到保存不良的胶原蛋白的转变中，胶原蛋白保存的最可靠指标是胶原蛋白中碳和氮的浓度。在这个转变点，C和N的浓度突然下降一个数量级。这些属性为评估样品质量提供了简单的标准。由于胶原蛋白的保存在史前遗址中差异很大，因此应报告每个标本的这些属性。建议使用净化程序去除酸和碱溶性污染物和颗粒物质（碳酸盐、富里酸、脂类、腐殖酸、沉积物和根）。更广泛地采用这些程序将确保实验室之间结果的可比性，并允许对样品保存进行独立和客观的评价，以及对胶原蛋白同位素分析进行更精确的饮食、气候和栖息地解释。
Criteria are presented for the identification of diagenetic alteration of carbon and nitrogen isotope ratios of bone and tooth collagen prepared by a widely used method. Measurements of collagen concentrations in tooth and bone, atomic C:N ratios, and carbon and nitrogen concentrations in collagen of 359 historic and prehistoric African humans, and modern and prehistoric East African non-human mammals are described. Carbon isotope ratios of collagen lipids from four bones are also presented. Compared to bone, whole teeth have significantly lower collagen concentrations, lower carbon and nitrogen concentrations in collagen, and similar C:N ratios. Carbon and nitrogen concentrations and C:N ratios are relatively constant over a wide range of collagen concentrations. However, prehistoric specimens with very low collagen concentrations have highly variable C:N ratios, very low carbon and nitrogen concentrations in collagen, and stable carbon and nitrogen isotope ratios unlike collagen. At the transition from well-preserved to poorly preserved collagen the most reliable indicator of collagen preservation is the concentration of carbon and nitrogen in collagen. Concentrations of C and N drop abruptly by an order of magnitude at this transition point. These attributes provide simple criteria for assessing sample quality. Since collagen preservation can vary greatly within prehistoric sites, these attributes should be reported for each specimen. Use of purification procedures that remove acid- and base-soluble contaminants and particulate matter (carbonates, fulvic acids, lipids, humic acids, sediments and rootlets) are recommended. Wider adoption of these procedures would insure comparability of results between laboratories, and permit independent and objective evaluation of sample preservation, and more precise dietary, climatic, and habitat interpretations of collagen isotopic analyses.