Resolution of (R,S)‐ibuprofen catalyzed by immobilized Novozym40086 in organic phase
Resolution of (R,S)‐ibuprofen catalyzed by immobilized Novozym40086 in organic phase
复制标题
有机相中固定化 Novozym40086 催化拆分 (R,S) 布洛芬
作者：
袁欣;王璐骏;刘光勇;戴桂林;唐课文
以Novozym40086为催化剂，在有机溶剂中成功进行了布洛芬的对映选择性酯化反应。在反应混合物中加入去水剂，去除酯化过程中产生的水。考察了温度、正己醇浓度、布洛芬浓度和酶的负载等因素的影响。在平衡条件下，得到了热力学平衡常数K为7.697，对映选择性E为8.512。酯化反应在约30小时内达到平衡，转化率为56%，ees8为93.78%。X和ees的预测值分别为67.90%和95.60%。实验值与理论值近似相等，表明了理想模型的可行性。
The enantioselective esterification of ibuprofen catalyzed by Novozym40086 was successfully conducted in organic solvent. Removing‐water reagent was added into the reaction mixture to remove water produced in the esterification. The effects of temperature,n‐hexanol concentration, ibuprofen concentration, and loading of enzymes were investigated. Under the condition of equilibrium, the thermodynamic equilibrium constant (K) of 7.697 and enantioselectivity (E) of 8.512 were obtained. The esterification reaction achieved its equilibrium in approximately 30 hours with conversion of 56% and eeSof 93.78%. The predicted values of X and eeSwere 67.90% and 95.60%, respectively. The experimental value is approximately equal to the theoretical value, which indicates the feasibility of ideal models.