The use of fluorophore-containing spin traps as potential probes to localize free radicals in cells with fluorescence imaging methods.
The use of fluorophore-containing spin traps as potential probes to localize free radicals in cells with fluorescence imaging methods.
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使用含有荧光团的自旋陷阱作为潜在探针，通过荧光成像方法定位细胞中的自由基。
DOI：
10.1096/fasebj.9.11.7649408
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Rosen,GM
中科院分区：
文献类型：
--
作者：
Pou,S;Bhan,A;Bhadti,VS;Wu,SY;Hosmane,RS;Rosen,GM
自由基过程研究的核心是识别和定位其细胞形成位点的能力。在最好的实验条件下，自旋捕获/ESR光谱只能表征细胞内产生的特定自由基和共聚焦显微镜只能定位其形成的网站。在这篇文章中，我们报告了一种含荧光团的硝酮，α-[4-[5-（（2-羧基）苯基）-5-羟基-4-氧代-3-苯基）-2-吡咯烷-1-基]苯基]-N-（叔丁基）硝酮钠盐（4）的发展。3该硝酮（4）与α-羟乙基自由基反应，二级速率常数为1.7 X 105 M 1 s-1，得到特征ESR光谱。然而，我们无法降低荧光发射，部分原因是α-羟乙基自由基与硝酮反应产生的氮氧化合物浓度较低（4）。使用含荧光团的氮氧自由基（7）作为模型，我们发现仅需要还原12%的氮氧自由基就可以使（7）的荧光发射增加近400%。我们的研究结果提出了开发各种含荧光团的硝酮的新方法，这些硝酮可以表征特定的自由基并定位其细胞内形成的位点。Pou，S.，巴恩，巴蒂，VS，Wu，S.是的，霍斯马内河美国，罗森，G. M.使用含荧光团的自旋陷阱作为潜在探针，通过荧光成像方法定位细胞中的自由基。FASEB J.9，1085 - 1090（1995）
Central to the study of free radical processes is the ability to identify and localize their cellular site of formation. Under the best of experimental conditions, spin trapping/ESR spectroscopy can only characterize intracellular production of specific free radicals and confocal microsocopy can only localize the site of their formation. In this article, we report on the development of a fluorophore‐containing nitrone, α‐[4‐[5‐((2‐carboxy)phenyl)‐5‐hydroxy‐4‐oxo‐3‐phenyl)‐2‐pyrroHn‐1 ‐yl]phenyl]‐N‐(lert‐butyl)nitrone sodium salt (4).3 This nitrone (4) reacts with α‐hydroxyethyl radical with a second order rate constant of 1.7 X 105 M 1 s‐1 to give a characteristic ESR spectrum. However, we were unable to decrease the fluorescence emission, due in part to the small concentration of nitroxide generated from the reaction of α‐hydroxyethyl radical with nitrone (4). Using the fluorophore‐containing nitroxide (7) as a model, we found that only 12% of the nitroxide needs to be reduced to give an almost 400% increase in the fluorescent emission of (7). Our findings suggest new approaches to the development of various fluorophore‐containing nitrones that can both characterize specific free radicals and localize their site of intracellular formation.—Pou, S., Bhan, A., Bhadti, V. S., Wu, S. Y., Hosmane, R. S., Rosen, G. M. The use of fluorophore‐containing spin traps as potential probes to localize free radicals in cells with fluorescence imaging methods.FASEB J.9, 1085‐1090 (1995)