Measurement of total, monomeric and polymeric IgA in human faeces by electroimmunodiffusion.
Measurement of total, monomeric and polymeric IgA in human faeces by electroimmunodiffusion.
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通过电免疫扩散法测量人类粪便中的总 IgA、单体 IgA 和聚合 IgA。
DOI：
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发表时间：
1987
影响因子：
4.6
通讯作者：
J. Gobert
中科院分区：
文献类型：
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作者：
D. Meillet;D. Raichvarg;F. Tallet;J. Šavel;J. Yonger;J. Gobert
使用改良的一维免疫电泳测定法同时测量连续三天收集的混合粪便材料中的总免疫球蛋白 A (IgA)、单体免疫球蛋白 A (IgA) 和聚合免疫球蛋白 A (P) 水平，这是一种精确且可重复的方法。 32 名腹泻患者的粪便 t-、m- 和 p-IgA 水平在统计学上高于 16 名健康受试者（分别是正常人的 2.2、2.6 和 2.0 倍）。当结果表示为每天的输出量以估计肠道的分泌能力时，这些值也显着不同（P小于0.001）。此外，腹泻患者粪便中的 m-IgA/t-IgA 百分比（42.3%）显着高于正常粪便（26.5%）。这些结果证实了粪便 IgA 测定对于估计各种胃肠道疾病中 IgA 局部产生的重要性。
Total (t), monomeric (m) and polymeric (p) immunoglobulin A (IgA) levels were simultaneously measured in mixed faecal material collected over three consecutive days using a modified one-dimensional immunoelectrophoretic assay, which is a precise and reproducible method. Faecal t-, m- and p-IgA levels were statistically higher in 32 diarrhoeic patients than in a group of 16 healthy subjects (respectively 2.2, 2.6 and 2.0 times the normal). The values were also significantly different (P less than 0.001) when results were expressed as output per day in order to estimate the secreting capacity of gut. Moreover, the m-IgA/t-IgA percentages were significantly higher in faeces from diarrhoeic patients (42.3%) than in normal faeces (26.5%). These results confirm the importance of faecal IgA determination in estimating the local production of the IgA in various gastrointestinal tract disorders.