Electroformation of giant liposomes from spin-coated films of lipids
Electroformation of giant liposomes from spin-coated films of lipids
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DOI：
10.1016/j.colsurfb.2005.01.016
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发表时间：
2005-05-10
影响因子：
5.8
通讯作者：
Mayer, M
中科院分区：
文献类型：
--
作者：
Estes, DJ;Mayer, M
本文介绍了脂质溶液的自旋涂覆，并利用所得到的薄膜电生成巨大的脂质体。旋转涂层使得在氧化铟锡的大表面（> 25 cm(2)）上生成厚度可控的均匀脂质膜成为可能。建立了电形成的最佳厚度范围（25-50 nm），我们证明了由脂质（如聚脂素、磷脂酰丝氨酸和磷脂酰甘油）形成的巨型脂质体，而传统的液滴衍生膜不容易形成巨型脂质体。我们比较了自旋涂覆脂质膜的脂质体与传统滴状衍生膜形成的脂质体，发现自旋涂覆膜比滴状衍生脂质膜产生更大（2-5倍）和更丰富的脂质体。最佳厚度的自旋涂覆均匀脂质膜的电形成提供了一种可重复的方法，可以在整个地层表面上获得直径主要大于30pm的脂质体。(c) 2005 Elsevier B.V.版权所有
This paper describes spin-coating of solutions of lipids and using the resulting thin films for electroformation of giant liposomes. Spin-coating made it possible to generate uniform films of lipids with controllable thickness over large surfaces ( > 25 cm(2)) of indium tin oxide. Establishing a range of thicknesses optimal for electroformation (25-50 nm), we demonstrate formation of giant liposomes from lipids (such as asolectin, phosphatidylserine, and phosphatidylglycerol) that do not readily form giant liposomes from traditional, droplet-derived films. We compared liposomes from a spin-coated film of lipids to liposomes formed from traditional droplet-derived films and found that spin-coated films produced larger (by factor of 2-5) and more abundant liposomes than droplet-derived films of lipids. Electroformation from spin-coated, homogenous lipid films of optimal thickness provided a reproducible way to obtain liposomes with diameters that are predominantly larger than 30 pm over the entire surface of formation. (c) 2005 Elsevier B.V. All rights reserved.