Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines.
Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines.
复制标题
在悬浮细胞系中鉴定编码长非编码RNA的潜在微肽的多核糖体分析和定量PCR。
DOI：
10.1016/j.xpro.2021.101037
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Han C;Sun L;Pan Q;Sun Y;Wang W;Chen Y
微肽正在成为各种细胞过程的重要调节因子。长的非编码RNA(LncRNAs)是编码微肽的小阅读框的来源。检测微肽或翻译lncRNA的技术，如质谱仪和核糖体图谱，既复杂又昂贵。在这里，我们提出了一种简单且经济有效的方案，通过在悬浮细胞系中的多聚体图谱来筛选潜在的微肽编码的lncRNA。当与定量聚合酶链式反应相结合时，该方法有助于同时识别多个翻译的lncRNA。有关本议定书的使用和执行的完整细节，请参阅。一种快速检测潜在的微肽编码的lncRNAs的方法详细描述了一个完整的多聚体图谱，提供了一种区分具有低或高翻译活性的RNA的方法。微肽正在成为各种细胞过程的重要调节因子。长的非编码RNA(LncRNAs)是编码微肽的小阅读框的来源。检测微肽或翻译lncRNA的技术，如质谱仪和核糖体图谱，既复杂又昂贵。在这里，我们提出了一种简单且经济有效的方案，通过在悬浮细胞系中的多聚体图谱来筛选潜在的微肽编码的lncRNA。当与定量聚合酶链式反应相结合时，该方法有助于同时识别多个翻译的lncRNA。
Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously. For complete details on the use and execution of this protocol, please refer to. A protocol for rapid detection of the potential micropeptide-encoding lncRNAs Detail a complete schedule for polysome profiling Provide a protocol to distinguish RNAs with low or high translation activity Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously.
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影响因子：
64.8
作者：
Thermann, Rolf;Hentze, Matthias W.
通讯作者：
Hentze, Matthias W.
DOI：
10.1083/jcb.71.3.933
发表时间：
1976-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Johnson LF;Levis R;Abelson HT;Green H;Penman S
通讯作者：
Penman S
影响因子：
14.8
作者：
Schneider-Poetsch, Tilman;Ju, Jianhua;Eyler, Daniel E.;Dang, Yongjun;Bhat, Shridhar;Merrick, William C.;Green, Rachel;Shen, Ben;Liu, Jun O.
通讯作者：
Liu, Jun O.
影响因子：
4.8
作者：
Halbeisen, Regula E.;Scherrer, Tanja;Gerber, Andre P.
通讯作者：
Gerber, Andre P.
影响因子：
4.5
作者：
Klein, DJ;Moore, PB;Steitz, TA
通讯作者：
Steitz, TA