A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization
A rhodamine-based fluorescent probe for Hg2+ and its application for biological visualization
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DOI：
10.1016/j.snb.2014.06.104
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发表时间：
2014-11-01
影响因子：
8.4
通讯作者：
Uvdal, Kajsa
中科院分区：
文献类型：
--
作者：
Hu, Jiwen;Hu, Zhangjun;Uvdal, Kajsa
通过在罗丹明B酰肼上添加羟甲基吡啶，合成了一种新的可见光激发荧光探针(1)。该探针在水体系中显示出非常特异的Hg2+诱导的变色和荧光增强。荧光的“开启”响应是基于罗丹明B中从螺内酰胺(非荧光)到无环黄杂菲(荧光)的结合诱导开环过程。羟甲基吡啶和罗丹明B肼的配位原子O中心点-N-N-O中心点在形成Hg2+化学计量比为1：1的络合物中起主导作用。它在0.1-5微米范围内呈线性响应，检测下限为15.7毫微米(3西格玛/斜率)。Hg2+/1缔合常数的计算值为0.70×10~(5)M~(-1)。此外，共聚焦显微镜成像实验表明，探针1可以作为荧光探针用于显示活的HeLa细胞中的Hg2+。(C)2014爱思唯尔B.V.保留所有权利。
A new visible light excitable fluorescent probe (1) is synthesized by appending a hydroxymethyl-pyridine to rhodamine B hydrazide. The probe displays very specific Hg2+-induced colour change and fluorescent enhancement in the aqueous systems. The "turn-on" response of fluorescence is based on a binding-induced ring-opening process from the spirolactam (nonfluorescent) to acyclic xanthene (fluorescent) in rhodamine B. The coordinating atoms O-center dot-N-N-O-center dot from the hydroxymethyl-pyridine and rhodamine B hydrazide play dominant role in the formation of a complex with 1:1 stoichiometry of Hg2+ to 1. It exhibits a linear response in the range of 0.1-5 mu M with the limit of detection (LOD) of 15.7 nM (3 sigma/slope), while the calculated value of the association constant of Hg2+/1 is 0.70 x 10(5) M-1. Furthermore, confocal microscopy imaging experiment demonstrates the probe 1 can be applied as a fluorescent probe for visualization of Hg2+ in living HeLa cells. (C) 2014 Elsevier B.V. All rights reserved.