Free radical scavenging and radioprotective effects of carnosine and anserine
Free radical scavenging and radioprotective effects of carnosine and anserine
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DOI：
10.1016/j.radphyschem.2009.07.023
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发表时间：
2009-12-01
影响因子：
2.9
通讯作者：
Hatano, Yoshihiko
中科院分区：
文献类型：
--
作者：
Fu, Haiying;Katsumura, Yosuke;Hatano, Yoshihiko
两种含组氨酸的天然二肽，肌肽和鹅氨酸（β-丙氨酰-1-甲基-L-组氨酸），已对其抗氧化和辐射防护能力进行了检查。脉冲辐射分解研究表明肌肽和鹅肌肽水溶液在不同 pH 值下具有抗氧化特性。在中性pH下，CH自由基与肌肽的反应速率常数在300 nm处确定为5.3 x 10(9) M-1 s(-1)，在400 nm处分别为4.1 x 10(9) M-1 s(-1)。琼脂糖凝胶电泳研究表明，肌肽和鹅肽还可以保护质粒 pUC18 DNA 免受 X 射线辐射引起的链断裂，肌肽在实验条件下表现出更高的保护效率。我们的数据表明肌肽和鹅氨酸可能在维持抗氧化系统中发挥重要作用。 (C) 2009 Elsevier Ltd. 保留所有权利。
Two histidine-containing natural dipeptides, carnosine and anserine (beta-alanyl-1-methyl-L-histidine), have been examined for their antioxidant and radioprotective abilities. Pulse radiolysis studies indicated the antioxidative properties of carnosine and anserine aqueous solutions at different pH. The rate constants for the reaction CH radical with carnosine at neutral pH were determined to be 5.3 x 10(9) M-1 s(-1) at 300 nm, and 4.1 x 10(9) M-1 s(-1) at 400 nm, respectively. Carnosine and anserine also protected plasmid pUC18 DNA from X-ray radiation-induced strand breaks as evidenced from the studies by agarose gel electrophoresis, Carnosine showed higher protective efficiency under the experimental conditions. Our data demonstrated that carnosine and anserine may play an important role in the maintenance of the antioxidant system. (C) 2009 Elsevier Ltd. All rights reserved.