Effects of developmental exposure to ethanol on Caenorhabditis elegans
Effects of developmental exposure to ethanol on Caenorhabditis elegans
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DOI：
10.1111/j.1530-0277.2008.00639.x
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发表时间：
2008-05-01
影响因子：
3.2
通讯作者：
Rankin, Catharine H.
中科院分区：
文献类型：
--
作者：
Davis, Justin R.;Li, Yuri;Rankin, Catharine H.
背景：我们调查了慢性酒精暴露对微小线虫线虫的身体发育、生殖和预期寿命的影响。它有一个由302个神经元组成的小神经系统，寿命很短，只有2-3周。方法：在本研究中，这些蠕虫在不同的生命阶段长期暴露于不同浓度的乙醇中：整个生命周期，仅在幼虫发育期间，仅在成虫发育期间。此外，在胚胎发育的不同阶段，幼虫还被急性暴露于乙醇。结果：幼虫发育期间长期暴露于乙醇会暂时延缓体格生长，延缓发育，推迟生殖成熟的开始，并降低生殖繁殖力和寿命。当蠕虫完成发育并达到生殖成熟时，长期接触乙醇会导致线虫体长缩短，生殖繁殖力和预期寿命下降。最后，线虫卵在不同发育阶段急性暴露于高浓度乙醇的胚胎中，根据发育过程中卵子暴露的时间，暴露的卵孵化成幼虫的可能性较低。此外，由于胚胎发育期间急性乙醇暴露，一些孵化的蠕虫显示出明显的物理畸形。结论：这些数据表明，在关键发育时期将线虫暴露在乙醇中会导致特有的表型结果。因此，秀丽线虫为探索酒精暴露影响发育的机制提供了一个新的模型。
Background: We investigated the effects of chronic ethanol exposure on physical development, reproduction, and life expectancy of Caenorhabditis elegans, a microscopic nematode worm. It has a small nervous system of 302 neurons and a short lifespan of 2 to 3 weeks.Methods: In this study, the worms were chronically exposed to varying concentrations of ethanol for different periods of their life: for their entire lifespan, during larval development only, and during adulthood only. In addition, the worms were exposed to ethanol acutely during different stages of embryonic development.Results: Chronic exposure to ethanol during larval development temporarily delayed physical growth, slowed development, delayed the onset of reproductive maturity, and decreased both reproductive fecundity and longevity. Chronic exposure to ethanol beginning when worms completed development and reached reproductive maturity resulted in reduced C. elegans body length, decreased reproductive fecundity, and life expectancy. Finally, acute embryonic exposure of C. elegans eggs to high concentrations of ethanol at different stages of development resulted in a lower probability of exposed eggs hatching into larval worms depending on when eggs were exposed during development. Furthermore, some of the worms that did hatch displayed distinct physical dysmorphologies as a consequence of acute ethanol exposure during embryonic development.Conclusions: These data suggest that exposing C. elegans to ethanol during critical development periods results in characteristic phenotypic outcomes. Thus, C. elegans offers a novel model for exploring the mechanisms by which ethanol exposure affects development.