Induction of meandering functional reentrant wave front in isolated human atrial tissues.
Induction of meandering functional reentrant wave front in isolated human atrial tissues.
复制标题
在孤立的人体心房组织中诱导蜿蜒的功能性折返波前。
DOI：
10.1161/01.cir.96.9.3013
复制
发表时间：
1997
期刊：
影响因子：
37.8
通讯作者：
Chen,PS
中科院分区：
文献类型：
--
作者：
Ikeda,T;Czer,L;Trento,A;Hwang,C;Ong,JJ;Hough,D;Fishbein,MC;Mandel,WJ;Karagueuzian,HS;Chen,PS
BackgroundThe目的是本研究是为了测试的假设，一个单一的曲折功能折返波前可以导致在快速和不规则的电图活动在人类atrial tissues.Methods和ResultsThe研究使用了5个人类心脏移植受体的心脏。切除3个右心房和2个左心房组织样本，厚度为3.4±0.3 mm，并修剪至3.5×3.0 cm。将分离的心房置于具有477双极记录电极阵列的腔室中，该双极记录电极阵列内置于组织浴的底部。电极间距离为1.6 mm。组织以10 mL/min的速率持续灌注36.5°C含氧台氏液。在从组织边缘以400或600 ms周期长度提供8次基线刺激（S1）后，在组织中心给予单一过早刺激（S2）以诱导折返。共诱发9次折返，S1-S2耦合间期为232±29 ms（范围：190 - 290 ms），S2强度为10±3 mA（范围：5 - 15 mA）。在所有样本中，诱导了单个弯曲折返波前，导致不规则和快速双极电描记图活动。这些波前的平均周期长度为229±45 ms（160 ~ 290 ms），持续时间为1.1±0.3 s（0.6 s ~ 2.5 s）或5.2±1.4（3 ~ 9）个周期，然后自发终止。
BackgroundThe purpose of this study was to test the hypothesis that a single meandering functional reentrant wave front can result in rapid and irregular electrogram activity in human atrial tissues.Methods and ResultsThe study used the explanted hearts of five human cardiac transplant recipients. Three right and two left atrial tissue samples, 3.4±0.3 mm thick, were excised and trimmed to 3.5×3.0 cm. The isolated atrium was placed endocardial surface down in a chamber with a 477 bipolar recording electrode array built into the bottom of the tissue bath. The interelectrode distance was 1.6 mm. The tissue was constantly superfused with 36.5°C oxygenated Tyrode’s solution at a rate of 10 mL/min. After eight baseline stimuli (S1) delivered at 400- or 600-ms cycle length from the edge of the tissue, a single premature stimulus (S2) was given at the center of the tissue to induce reentry. A total of nine episodes of reentry were induced with S1-S2coupling intervals of 232±29 ms (range, 190 to 290 ms) and an S2strength of 10±3 mA (range, 5 to 15 mA). In all samples, a single meandering reentrant wave front was induced, causing irregular and rapid bipolar electrogram activity. These wave fronts had a mean cycle length of 229±45 ms (160 to 290 ms) and persisted for 1.1±0.3 seconds (0.6 seconds to 2.5 seconds), or 5.2±1.4 (3 to 9) cycles, before spontaneous termination.ConclusionsA single meandering functional reentrant wave front can be induced in human atrial tissues and produce rapid and irregular electrical activity.