Effects of occupation, lifestyle and genetic polymorphisms of CYP1A1, CYP2E1, GSTM1 and GSTT1 on urinary 1-hydroxypyrene and 2-naphthol concentrations
Effects of occupation, lifestyle and genetic polymorphisms of CYP1A1, CYP2E1, GSTM1 and GSTT1 on urinary 1-hydroxypyrene and 2-naphthol concentrations
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DOI：
10.1093/carcin/22.5.787
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发表时间：
2001-05-01
期刊：
影响因子：
4.7
通讯作者：
Kwon, FH
中科院分区：
文献类型：
--
作者：
Nan, HM;Kim, N;Kwon, FH
本研究旨在确定职业、生活方式以及细胞色素P450 1A1 （CYP1A1）、细胞色素P450 2E1 （CYP2E1）和谷胱甘肽s -转移酶mu1 （GSTM1）和theta1 （GSTT1）基因多态性对韩国焦炉工人和大学生尿1-羟基芘（1-OHP）和2-萘酚浓度的影响。研究对象包括90名焦炉工人和128名大学生。通过问卷调查的方式获得了研究对象工作区域、吸烟习惯和食物摄入的详细数据。研究了尿中多环芳烃（PAH）浓度与职业、吸烟状况、空气中总PAH水平和遗传多态性的关系。焦炉工人尿液中1-OHP和2-萘酚浓度高于学生，且与工作区域显著相关。尿中2-萘酚浓度随学生吸烟程度的增加而增加。焦炉工人尿1-OHP浓度与空气中多环芳烃含量相关。CYP2E1 c1/c2或c2/c2基因型的焦炉工人尿液中1-OHP和2-萘酚浓度高于c1/c1基因型的工人，gstm1阴性的工人尿液中2-萘酚浓度高于gstm1阳性的工人，多元回归分析表明，CYP2E1是决定焦炉工人尿液中1-OHP浓度的重要因素。CYP2E1和GSTM1是焦炉工人尿中2-萘酚浓度的重要决定因素，GSTM1和吸烟是大学生尿中2-萘酚浓度的预测因子，尿中1-OHP是焦炉工人职业接触多环芳烃的较好指标，而尿中2-萘酚可能对非职业吸入接触多环芳烃更为敏感。在职业暴露人群中，CYP2E1和GSTM1似乎在芘和萘的代谢中起重要作用，在非职业暴露于多环芳烃的人群中，GSTM1和吸烟似乎影响尿中2-萘酚的浓度。
This study was undertaken to determine the effects of occupation, lifestyle and the genetic polymorphisms of cytochrome P450 1A1 (CYP1A1), cytochrome P450 2E1 (CYP2E1) and glutathione S-transferases mu1 (GSTM1) and theta1 (GSTT1) on the concentrations of urinary 1-hydroxypyrene (1-OHP) and 2-naphthol among Korean coke oven workers and university students. The study subjects included 90 coke oven workers and 128 university students. A questionnaire was used to obtain detailed data about the work area, smoking habits and food intake of subjects. Associations between urinary polycyclic aromatic hydrocarbon (PAH) concentrations and occupation, smoking status, total airborne PAH level and genetic polymorphisms were tested. Urinary 1-OHP and 2-naphthol concentrations were higher in coke oven workers than in students and correlated significantly with work area. Urinary 2-naphthol concentrations increased with an increase in the level of cigarette smoking in students. Total airborne PAH level correlated with urinary 1-OHP concentration in coke oven workers. Urinary 1-OHP and 2-naphthol concentrations were higher in coke oven workers with the c1/c2 or c2/c2 genotype of CYP2E1 than in those with the c1/c1 genotype, Urinary 2-naphthol concentrations were higher in GSTM1-null workers than in GSTM1-positive workers, In multiple regression analysis CYP2E1 was a significant factor determining urinary 1-OHP concentrations in coke oven workers. CYP2E1 and GSTM1 were significant determinants for urinary 2-naphthol concentrations in coke oven workers and GSTM1 and smoking were prognosticators among university students, Urinary 1-OHP is a better indicator of occupational exposure to PAH in coke oven workers than 2-naphthol, whereas urinary 2-naphthol may be more sensitive for non-occupational inhalation exposure to PAH. In occupationally exposed populations CYP2E1 and GSTM1 appear to play an important role in the metabolism of pyrene and naphthalene, In individuals not occupationally exposed to PAHs GSTM1 and smoking seem to influence the urinary concentration of 2-naphthol.