RANKL upregulation associated with periodontitis and Porphyromonas gingivalis.
RANKL upregulation associated with periodontitis and Porphyromonas gingivalis.
复制标题
RANKL 上调与牙周炎和牙龈卟啉单胞菌相关。
DOI：
10.1902/jop.2007.060398
复制
发表时间：
2007
影响因子：
4.3
通讯作者：
W. Pitiphat
中科院分区：
文献类型：
--
作者：
N. Wara;R. Surarit;Anek Chayasadom;J. A. Boch;W. Pitiphat
背景
核因子-κ B受体激活因子（NF-κ B）配体（RANKL）和骨保护素（OPG）是维持破骨细胞活性的关键，提示它们在牙周炎骨丢失的进展中起重要作用。本研究探讨RANKL和OPG mRNA在牙周组织中的表达及其与牙周致病菌的关系。
方法
收集15例慢性牙周炎患者和15例牙周健康者的牙龈组织和龈下菌斑样本。从组织中提取RNA，并使用RANKL或OPG特异性引物进行逆转录-聚合酶链反应（RT-PCR）。将β-肌动蛋白作为对照扩增以确保相等的加载。通过光密度计与β-肌动蛋白的强度成比例地分析RT-PCR产物的强度。实时荧光定量PCR检测牙龈卟啉单胞菌和伴放线放线杆菌的数量。
结果
我们的研究结果显示RANKL mRNA在慢性牙周炎组织中的水平增加。牙周炎组RANKL/OPG表达率显著高于健康对照组（P = 0.001）。有趣的是，RANKL（r = 0.64; P <0.001）而非OPG（r = -0.24; P = 0.20）的表达与牙龈卟啉单胞菌数量的增加显著相关。A.仅6.7%的点检出伴放线菌。
结论
慢性牙周炎与RANKL mRNA表达上调和RANKL/OPG mRNA表达比值增加有关。此外，据我们所知，我们的数据首次显示了RANKL水平上调与临床获得的牙周组织中牙龈卟啉单胞菌数量之间的相关性。
BACKGROUND
Receptor activator of nuclear factor-kappa B (NF-kappaB) ligand (RANKL) and osteoprotegerin (OPG) are critical for homeostatic control of osteoclast activity, suggesting their vital roles in the progression of bone loss in periodontitis. In this study, the expression of RANKL and OPG mRNA and the relationship between these factors and periodontopathic bacteria in periodontal tissue were studied.
METHODS
Gingival tissue and subgingival plaque samples were collected from 15 patients with chronic periodontitis and 15 periodontally healthy subjects. RNA was extracted from the tissue and subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primers specific for RANKL or OPG. Beta-actin was amplified as a control to ensure equal loading. The intensity of RT-PCR products was analyzed by a densitometer in proportion to the intensity of beta-actin. The numbers of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans were determined by quantitative real-time PCR.
RESULTS
Our results showed increased levels of RANKL mRNA in chronic periodontitis tissues. The RANKL/OPG expression ratio was significantly higher in the periodontitis group compared to the healthy control group (P = 0.001). Interestingly, the expression of RANKL (r = 0.64; P <0.001), but not OPG (r = -0.24; P = 0.20), was significantly correlated with increased numbers of P. gingivalis. A. actinomycetemcomitans was detected in only 6.7% of all sites.
CONCLUSIONS
Chronic periodontitis was associated with RANKL mRNA upregulation and increased RANKL/OPG mRNA expression ratio. In addition, our data showed for the first time to our knowledge an association between upregulated RANKL levels and the number of P. gingivalis in clinically obtained periodontal tissues.
DOI：
10.1172/jci23394
发表时间：
2005-02
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
S. Wei;H. Kitaura;P. Zhou;F. Ross;S. Teitelbaum
通讯作者：
S. Wei;H. Kitaura;P. Zhou;F. Ross;S. Teitelbaum
影响因子：
15.9
作者：
Teng, YTA;Nguyen, H;Penninger, JM
通讯作者：
Penninger, JM