Evaluation of the Nonpathogenic Agrobacterium vitis Strain ARK-1 for Crown Gall Control in Diverse Plant Species
Evaluation of the Nonpathogenic Agrobacterium vitis Strain ARK-1 for Crown Gall Control in Diverse Plant Species
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DOI：
10.1094/pdis-06-14-0588-re
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发表时间：
2015-03-01
期刊：
影响因子：
4.5
通讯作者：
Tanina, Koji
中科院分区：
文献类型：
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作者：
Kawaguchi, Akira;Inoue, Koji;Tanina, Koji
葡萄根瘤菌（Agrobacterium葡萄属）方舟-1是一种能有效防治葡萄藤根癌病的生物制剂。在这项研究中，处理苹果，日本梨，桃，玫瑰和番茄的根浸泡在细胞悬浮液的方舟-1种植到土壤中的致瘤农杆菌感染。减少了植物冠瘿瘤的数量。对2010年至2013年的6个苹果田间试验、4个日本梨田间试验和4个桃田间试验结果的荟萃分析显示，方舟-1处理后，苹果冠瘿的综合风险比（IRR）为0.38，日本梨冠瘿为0.16，桃冠瘿为0.20，表明方舟-1处理显著降低了发病率。对3个温室玫瑰试验和3个温室番茄试验结果的Meta分析表明，方舟-1处理后玫瑰冠瘿病的IRR为0.29，番茄冠瘿病的IRR为0.16，表明方舟-1处理显著降低了发病率。这些结果表明，方舟-1的控制覆盖了五种不同的宿主植物和农杆菌属物种的致瘤（Ti）菌株。
The nonpathogenic strain of Agrobacterium (=Rhizobium) vitis ARK-1 is a biological agent able to effectively control grapevine crown gall. In this study, treating apple, Japanese pear, peach, rose, and tomato by soaking the roots in a cell suspension of ARK-1 before planting into soil infected with tumorigenic Agrobacterium spp. reduced the number of plants developing crown gall tumors. Meta-analyses of the results from six field trials of apple, four field trials of Japanese pear, and four field trials of peach, from 2010 to 2013, showed integrated risk ratio (IRR) after treatment with ARK-1 to be 0.38 for apple crown gall, 0.16 for Japanese pear crown gall, and 0.20 for peach crown gall, indicating that the disease incidence was significantly reduced by ARK-1 treatment. Meta-analyses of the results from three greenhouse trials of rose and three greenhouse trials of tomato showed IRR after treatment with ARK-1 to be 0.29 for rose crown gall and 0.16 for tomato crown gall, indicating that the disease incidence was significantly reduced by ARK-1 treatment. These results indicated that control by ARK-1 covers five different species of host plants and tumorigenic (Ti) strains of Agrobacterium species.