Similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates
Similarity of tetracycline resistance genes isolated from fish farm bacteria to those from clinical isolates
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DOI：
10.1128/aem.69.9.5336-5342.2003
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发表时间：
2003-09-01
影响因子：
4.4
通讯作者：
Ohtal, M
中科院分区：
文献类型：
--
作者：
Furushita, M;Shiba, T;Ohtal, M
2000年8月和9月，从日本南部三个不同养鱼场采集的鱼类中分离出四环素耐药（Tet(r)）细菌。66株革兰氏阴性Tet(r)菌株中，29株仅携带tetB。四个携带tetY，另外四个携带tetD。3株携带tetC， 2株携带tetB和tetY， 1株携带tetC和tetG。序列分析表明，鱼场菌与临床菌的Tet(r)基因同源性为：tetB为99.3% ~ 99.9%，tetC为98.2% ~ 100%，MID为99.7% ~ 100%，tetG为92.0 ~ 96.2%，tetY为97.1 ~ 100%。11株Tet(r)菌株通过接合将Tet(r)基因转移到大肠杆菌HB-101上。所有转偶联物对四环素、氧环素、强力环素和米诺环素均有耐药性。供体包括光杆菌、弧菌、假单胞菌、互变单胞菌、柠檬酸杆菌和沙门氏菌，它们将tetB、tetY或tetD转移给受体。由于NaCl能促进Tet(r)菌株的生长，除假单胞菌（Pseudomonas）、柠檬酸杆菌（Citrobacter）和沙门氏菌（Salmonella）外，其余的Tet(r)菌株被认为是海洋细菌。我们的结果表明，来自养鱼场细菌的tet基因与来自临床菌株的tet基因具有相同的起源。
Tetracycline-resistant (Tet(r)) bacteria were isolated from fishes collected at three different fish farms in the southern part of Japan in August and September 2000. Of the 66 Tet(r) gram-negative strains, 29 were identified as carrying tetB only. Four carried tetY, and another four carried tetD. Three strains carried tetC, two strains carried tetB and tetY, and one strain carried tetC and tetG. Sequence analyses indicated the identity in Tet(r) genes between the fish farm bacteria and clinical bacteria: 99.3 to 99.9% for tetB, 98.2 to 100% for tetC, 99.7 to 100% for MID, 92.0 to 96.2% for tetG, and 97.1 to 100% for tetY. Eleven of the Tet(r) strains transferred Tet(r) genes by conjugation to Escherichia coli HB-101. All transconjugants were resistant to tetracycline, oxycycline, doxycycline, and minocycline. The donors included strains of Photobacterium, Vibrio, Pseudomonas, Alteromonas, Citrobacter, and Salmonella spp., and they transferred tetB, tetY, or tetD to the recipients. Because NaCl enhanced their growth, these Tet(r) strains, except for the Pseudomonas, Citrobacter, and Salmonella strains, were recognized as marine bacteria. Our results suggest that tet genes from fish farm bacteria have the same origins as those from clinical strains.