Characterization of the quantitative trait locus OilA1 for oil content in Brassica napus
Characterization of the quantitative trait locus OilA1 for oil content in Brassica napus
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甘蓝型油菜含油量数量性状位点 OilA1 的表征
DOI：
10.1007/s00122-013-2150-5
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发表时间：
2013-10-01
影响因子：
5.4
通讯作者：
Zhao, Jianyi
中科院分区：
文献类型：
--
作者：
Chen, Yubo;Qi, Lu;Zhao, Jianyi
提高油菜种子含油量已成为油菜育种的重要指标之一。然而，含油量是一个复杂的数量性状。利用德国Sollux和中国高邮品种杂交的双单倍体群体（SG群体）进行QTL定位，发现一个油分含量QTL位于连锁群A1（OilA 1）上，遗传距离为17 cM。为了进一步验证和表征OilA 1，我们使用B构建了一个高分辨率地图。以DH系SG-DH 267为供体，以中国亲本高邮为轮回背景，构建了一套近等基因系（nearisogenic lines，NILs）。结果表明，B之间的同线性顺序高度保守。rapa和B.在连锁群A1中发现了一个位于两个标记ZAASA 1 -38和NTP 3之间的可能的着丝粒区域（2.5cM）。OilA 1首先通过250株BC 5 F2植物验证，并在标记ZAASA 1 -47和ZAASA 1 -77之间的10.6cM间隔中得到证实。利用2代QTL近等基因系、8个BC 5 F3：4家系的283个株系和6个BC 5 F4亚近等基因系的428个单株进行了进一步的代换作图，将OilA 1的代换区间分别缩小到6.9 cM和4.3 cM（1.4 Mb）。利用纯合的BC 5 F3：4姊妹亚近等基因系进行的田间调查表明，携带Sollux染色体片段的近等基因系的含油量（1.26%，p < 0.001）显著高于其含有高邮染色体的姊妹近等基因系。OilA 1基因座在中国的育种目的中特别感兴趣，因为80%的中国栽培品种不携带该理想的等位基因。
Increasing seed oil content has become one of the most important breeding criteria in rapeseed (Brassica napus). However, oil content is a complex quantitative trait. QTL mapping in a double haploid population (SG population) emerging from a cross between a German (Sollux) and Chinese (Gaoyou) cultivars revealed one QTL for oil content on linkage group A1 (OilA1), which was mapped to a 17 cM genetic interval. To further validate and characterize the OilA1, we constructed a high-resolution map using B. rapa sequence resources and developed a set of near-isogenic lines (NILs) by employing a DH line SG-DH267 as donor and Chinese parent Gaoyou as recurrent background. The results showed highly conserved synteny order between B. rapa and B. napus within the linkage group A1 and revealed a possible centromere region between two markers ZAASA1-38 and NTP3 (2.5 cM). OilA1 was firstly validated by 250 BC5F2 plants and was confirmed in a 10.6 cM interval between the markers ZAASA1-47 and ZAASA1-77. Further substitution mapping was conducted by using two generations of QTL-NILs, 283 lines from eight BC5F3:4 families and 428 plants from six BC5F4 sub-NILs and thus narrowed the OilA1 interval to 6.9 cM and 4.3 cM (1.4 Mb), respectively. Field investigations with two replications using homozygous BC5F3:4 sister sub-NILs indicated that NILs, which carry a Sollux chromosome segment across the target region showed significant higher oil content (1.26 %, p < 0.001) than their sister NILs containing Gaoyou chromosome. The OilA1 locus is of particular interest for breeding purpose in China because 80 % of Chinese cultivars do not carry this desirable allele.