The plant response to high CO2 levels is heritable and orchestrated by DNA methylation
The plant response to high CO2 levels is heritable and orchestrated by DNA methylation
复制标题
植物对高二氧化碳浓度的反应是可遗传的，并由 DNA 甲基化协调
DOI：
10.1111/nph.18876
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发表时间：
2023
期刊：
影响因子：
9.4
通讯作者：
Fahlgren, Noah
中科院分区：
文献类型：
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作者：
Panda, Kaushik;Mohanasundaram, Boominathan;Gutierrez, Jorge;McLain, Lauren;Castillo, S. Elizabeth;Sheng, Hudanyun;Casto, Anna;Gratacós, Gustavo;Chakrabarti, Ayan;Fahlgren, Noah
众所周知，植物对非生物环境挑战的反应除了最初的胁迫暴露之外，还会对植物产生持久的影响。其中一些持久影响是跨代的，影响下一代。植物对二氧化碳 (CO2) 水平升高的反应已得到充分研究。然而，这些研究通常仅限于在高 CO2 环境中生长的单代植物，而跨代研究很少。我们的目的是通过研究恢复到基线 CO2 水平时的直系后代来确定暴露于高 CO2 后植物的跨代生长反应。我们发现开花植物拟南芥和无籽非维管植物小立碗藓在暴露于高 CO2 的植物后代中继续表现出加速的生长速率。我们使用模式物种拟南芥来剖析其分子机制，发现DNA甲基化途径对于这种生长反应的遗传性是必需的。更具体地说，RNA引导的DNA甲基化途径是启动甲基化所必需的，而蛋白质CMT2和CMT3是这种DNA甲基化向后代植物跨代繁殖所必需的。这两条 DNA 甲基化途径共同建立并维持细胞对高二氧化碳暴露的记忆。
Plant responses to abiotic environmental challenges are known to have lasting effects on the plant beyond the initial stress exposure. Some of these lasting effects are transgenerational, affecting the next generation. The plant response to elevated carbon dioxide (CO2) levels has been well studied. However, these investigations are typically limited to plants grown for a single generation in a high CO2environment while transgenerational studies are rare.We aimed to determine transgenerational growth responses in plants after exposure to high CO2by investigating the direct progeny when returned to baseline CO2levels.We found that both the flowering plantArabidopsis thalianaand seedless nonvascular plantPhyscomitrium patenscontinue to display accelerated growth rates in the progeny of plants exposed to high CO2. We used the model species Arabidopsis to dissect the molecular mechanism and found that DNA methylation pathways are necessary for heritability of this growth response.More specifically, the pathway of RNA‐directed DNA methylation is required to initiate methylation and the proteins CMT2 and CMT3 are needed for the transgenerational propagation of this DNA methylation to the progeny plants. Together, these two DNA methylation pathways establish and then maintain a cellular memory to high CO2exposure.