Identification and characterization of nodulation-signaling pathway 2, a gene of Medicago truncatula involved in Nod actor signaling.
Identification and characterization of nodulation-signaling pathway 2, a gene of Medicago truncatula involved in Nod actor signaling.
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作者：
G. Oldroyd;S. Long
细菌来源的结瘤因子在豆科植物/根瘤菌共生关系的建立中是至关重要的。了解植物结瘤因子的感知和信号转导机制，将大大推进我们对这种复杂相互作用的理解。在这里，我们描述了一个新位点的鉴定，即参与结瘤因子信号传导的截形苜蓿结瘤信号通路2（NSP 2）。该位点的突变体被Nod因子诱导的基因表达阻断，并显示出降低的根毛变形反应。nsp 2植物在接种苜蓿中华根瘤菌后也显示完全没有感染和皮层细胞分裂。结瘤因子诱导的钙尖峰，测试的最早的反应之一，仍然是在这些突变体植物的功能。我们的结论是，基因NSP 2是一个组件的结瘤因子的信号转导通路，位于下游的钙尖峰反应。
Bacterially derived Nod factor is critical in the establishment of the legume/rhizobia symbiosis. Understanding the mechanisms of Nod factor perception and signal transduction in the plant will greatly advance our understanding of this complex interaction. Here, we describe the identification of a new locus, nodulation-signaling pathway 2 (NSP2), of Medicago truncatula that is involved in Nod factor signaling. Mutants at this locus are blocked for Nod factor-induced gene expression and show a reduced root hair deformation response. nsp2 plants also show a complete absence of infection and cortical cell division following Sinorhizobium meliloti inoculation. Nod factor-induced calcium spiking, one of the earliest responses tested, is still functional in these mutant plants. We conclude that the gene NSP2 is a component of the Nod factor signal transduction pathway that lies downstream of the calcium-spiking response.