Expression of heme oxygenase-1 in the lungs of rats exposed to crocidolite asbestos
Expression of heme oxygenase-1 in the lungs of rats exposed to crocidolite asbestos
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DOI：
10.1080/08958370590922580
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发表时间：
2005-05-15
影响因子：
2.1
通讯作者：
Tanaka, I
中科院分区：
文献类型：
--
作者：
Nagatomo, H;Morimoto, Y;Tanaka, I
氧化应激被认为是石棉所致肺纤维化的发病机制，血红素氧合酶-1（HO-1）对肺组织的氧化应激具有保护作用。我们假设HO-1与石棉暴露引起的氧化性肺损伤有关。本研究旨在探讨青石棉对肺组织HO-1表达的影响。雄性Wistar大鼠一次性腹腔内滴注青石棉1 mg或2 mg，分别于3d、1 wk、1 mo、3 mo和6 mo处死。Western blot和免疫组化染色观察HO-1的表达。腹腔内注入2 mg青石棉后3 ~ 6个月HO-1蛋白水平升高。HO-1水平在1 wk和1 mo时升高。在免疫染色中发现许多HO-1阳性细胞，特别是在肺泡巨噬细胞中。这些结果表明，HO-1可能与粉尘引起的肺疾病有关，因此可以作为粉尘暴露所致肺损伤的生物标志物。
Oxidative stress is thought to be the pathogenesis of pulmonary fibrosis induced by asbestos, and heme oxygenase-1 (HO-1) protects lung tissue against oxidative stress. We hypothesized that HO-1 is associated with oxidative lung injury caused by exposure to asbestos. This study was conducted to investigate the time course of HO-1 expression of lungs exposed to crocidolite asbestos in vivo. Male Wistar rats were administered 1 mg or 2 mg crocidolite asbestos suspended in saline by a single intratracheal instillation and were sacrificed at 3 d, 1 wk, 1 mo, 3 mo, and 6 mo of recovery time. The expression of HO-1 was observed by Western blot analysis and immunostaining. Protein levels of HO-1 increased at from 3 d to 6 mo following intratracheal instillation of 2 mg crocidolite asbestos. The levels of HO-1 increased at 1 wk and 1 mo following intratracheal instillation of 1 mg crocidolite asbestos. Many HO-1-positive cells were found, particularly in the alveolar macrophages, during immunostaining. These findings suggest that HO-1 may be related to lung disorder induced by dust and therefore can act as a biomarker of lung injury due to dust exposure.