Oral microbiome alterations of healthy volunteers with proton pump inhibitor
Oral microbiome alterations of healthy volunteers with proton pump inhibitor
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DOI：
10.1111/jgh.14040
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发表时间：
2018-05-01
影响因子：
4.1
通讯作者：
Kinoshita, Yoshikazu
中科院分区：
文献类型：
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作者：
Mishiro, Tsuyoshi;Oka, Kentaro;Kinoshita, Yoshikazu
背景和氨基酸抑制剂包括质子泵抑制剂(PPI)被用作各种酸相关性胃肠道疾病的一线治疗。虽然已知它们能显著减少胃酸的产生，但它们作为胃肠道微生物组功能的一部分，对抑酸分泌的影响仍有待阐明。本研究的目的是观察PPI的使用对健康志愿者口腔和肠道微生物区系的影响。在每天服用一次20毫克埃索美拉唑的4周前和4周后，我们分别从受试者的粪便、唾液和牙周袋液体中采集样本。结果唾液、牙周袋和粪便中细菌的物种丰富度(α多样性)存在显著差异。此外，对UniFrac距离的测量，尽管个体间存在差异(β多样性)，但唾液、牙周袋和粪便样本的微生物群结构明显彼此分开。唾液样本在服用PPI 4周前后的α和β多样性测量之间显示出显著差异。同时，基于分类群的分析表明，PPI管理增加了粪便样本中链球菌的比例，这表明潜在的不利影响导致肠道微生物区系改变。此外，随着细菌的过度生长(链球菌属)和不同细菌种类(奈瑟氏菌和韦洛氏菌)的减少，口腔运输微生物群中的微生物区系也发生了变化。
Background and AimAcid suppressive agents including proton pump inhibitors (PPIs) are used as first-line treatment for various acid-related gastrointestinal disorders. Although known to profoundly reduce gastric acid production, their influence on inhibition of acid secretion as part of the function of the gastrointestinal tract microbiome remains to be elucidated. The aim of the present study was to examine the effects of PPI usage on oral and gut microbiota in healthy volunteers.MethodsTen healthy adult volunteers receiving no medications were enrolled. We obtained fecal, saliva, and periodontal pocket fluid samples from the subjects before and after 4weeks of once daily administrations of 20-mg esomeprazole. The effects of PPI administration on bacterial communities were investigated using a 16S rRNA gene sequencing method.ResultsSpecies richness (alpha diversity) was significantly different among the salivary, periodontal pocket, and fecal samples. Furthermore, the measurements for UniFrac distances, despite inter-individual variations (beta diversity), of the microbiota structure of saliva and periodontal pocket and feces samples were clearly separated from each other. The salivary samples showed significant differences between alpha and beta diversity measurements before and after administration of the PPI for 4weeks. Meanwhile, taxon-based analysis indicated that PPI administration raised the ratio of Streptococcus organisms in fecal samples, suggesting a potentially unfavorable effect leading to gut microbiota alteration. Moreover, alterations of the microbiota in the oral carriage microbiome along with bacterial overgrowth (Streptococcus) and decreases in distinct bacterial species (Neisseria and Veillonella) were observed.ConclusionsThese results suggest that PPIs cause both oral and gut microbiota alterations.