Control of hepatocyte adhesion and function on self-organized honeycomb-patterned polymer film
Control of hepatocyte adhesion and function on self-organized honeycomb-patterned polymer film
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DOI：
10.1016/j.colsurfa.2005.11.098
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发表时间：
2006-08-15
影响因子：
5.2
通讯作者：
Shimomura, Masatsugu
中科院分区：
文献类型：
--
作者：
Tanaka, Masaru;Nishikawa, Kazutaka;Shimomura, Masatsugu
将肝细胞培养在自组织形成的蜂窝图案聚合物膜(蜂窝膜)上，以研究蜂窝图案对细胞行为的影响。用光学显微镜、荧光显微镜和扫描电子显微镜观察细胞形态和肌动蛋白细丝的变化。肝细胞扁平，肌动蛋白细丝明显出现在平片上的铺展区域。相反，在蜂窝膜上培养的肝细胞形成球状，肌动蛋白细丝位于球体的边缘内。在蜂窝薄膜上播种后数小时内可观察到球体，它们附着在一起，保持球体形状，不发生任何变形。与平膜上的细胞单层相比，球形细胞表达了更高水平的肝脏特异性功能。这些结果表明，蜂窝膜是一种适合于组织工程支架和生物医学装置的材料。(C)2005 Elsevier B.V.保留所有权利。
Hepatocytes were cultured on a honeycomb-patterned polymer film (honeycomb film) formed by self-organization in order to investigate the influence of the honeycomb pattern on cell behavior. The changes in cell morphologies and actin filaments were observed by optical, fluorescence, and scanning electron microscopy. Hepatocytes were flattened, and the actin filaments appeared conspicuously in the spreading regions on a flat film. In contrast, the hepatocytes that were cultured on the honeycomb film were observed to form a spherical shape, and the actin filaments were localized inside the edge of the spheroid. The spheroids were observed within several hours after seeding on the honeycomb film; they were attached and the spheroid shape was maintained without any deformation. The spheroids expressed a higher level of liver specific function than the cell monolayers on the flat film. These results suggest that the honeycomb film is a suitable material for tissue engineering scaffolds and biomedical devices. (c) 2005 Elsevier B.V. All rights reserved.